Biolegend, 616151, Purified anti-CD340 (ErbB2/HER-2) Antibody, 25μg

CD340 is the protein HER2, a member of the epidermal growth factor receptor (EGFR) family of cell membrane tyrosine kinases, and is encoded by the gene ErbB2. Unlike other EGFR family members, CD340 contains no ligand binding domain and has no known ligands. Instead, this protein interacts with other EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in adenocarcinoma of the lung, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. Overexpression of ErbB2 in breast cancers has been shown to confer Taxol resistance and is a therapeutic target in a number of human cancers.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human ErbB2, N-terminus
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICC - Quality tested FC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not recommended for use in IHC-P.
RRID: AB_3068205 (BioLegend Cat. No. 616151) AB_3068205 (BioLegend Cat. No. 616152)
Structure: CD340 is a 1,255 amino acid protein with a predicted molecular weight of 137.9 kD.
Distribution: Expressed on the cell surface of many tumor cells including some breast cancers, lung adenocarcinoma, gastric cancer, ovarian cancer, glioblastoma, some c-ALL blasts and bone marrow MSCs
Function: Contains no ligand binding domain and interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. Involved in embryonic development and cancer progression.
Interaction: CD340 interacts with a large number of proteins, including SHC1, EGF receptor, neuregulin 1, c-Src, integrin β4, Grb2, Grb7, SOS1, caveolin-1, JAK2, PAK1, FAK, and β-catenin, among others.
Ligand/Receptor: Despite being a member of the epidermal growth factor receptor (EGFR) family, no ligand of CD340 has been identified.
Cell Type: Leukemia
Biology Area: Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukocyte Biol. 74:593-601.
Gene ID: 2064
UniProt: View information about CD340 on UniProt.org
Clone: A20145B
Regulatory Status: RUO
Other Names: HER2/erbB2, tyrosine kinase cell surface receptor HER2, oncogene ERBB2, oncogene NGL, neuroblastoma- or glioblastoma-derived, HER-2
Isotype: Mouse IgG2b, κ