Biolegend, 616952, Purified anti-CD276 (B7-H3) Antibody, 100μg

CD276, or B7-H3, is a type I transmembrane protein and shares 20-27% amino acid identity with other B7 family members. Human CD276 has a single extracellular variable-type immunoglobulin (IgV)-IgC domain and a signature intracellular domain. There also exists an additional isoform known as 4Ig-B7-H3, containing a nearly exact tandem duplication of the IgV-IgC domain. Expression of CD276 is induced on T cells, natural killer (NK) cells, and antigen-presenting cells (APCs), including dendritic cells (DCs) and macrophages, and is upregulated during the maturation of monocytes to DCs, or following interactions between DCs and regulatory T cells. CD276 inhibits T-cell responses, and plays a role in adaptive immunity by suppressing T cell activation. CD276 is also expressed in various cancers, where it works to suppress tumor antigen-specific immune responses.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human CD276
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested FC, ICC, WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For flow cytometric staining, the suggested use of this reagent is ≤ 0.03 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Clone A20079E does not cross-react with mouse CD276 in WB.
RRID: AB_3068218 (BioLegend Cat. No. 616951) AB_3068218 (BioLegend Cat. No. 616952)
Structure: CD276 is a 534 amino acid protein with a predicted molecular weight of 58.2 kD.
Distribution: Dendritic cells, macrophages, natural killer cells, induced T cells. In the tonsil, it is expressed in epithelial cells.
Function: Co-stimulatory molecule that inhibits T-cell responses
Ligand/Receptor: CD276 binds to Trem-like transcript 2 (TLT-2).
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Inhibitory Molecules, Innate Immunity
Antigen References: Nagashima O, et al. 2008. J Immunol. 181:4062-71. Prasad DVR, et al. 2004. J Immunol. 173:2500-6. Sun M, et al. 2002. J Immunol. 168:6294-7. Xu J, et al. 2006. Cellular and Molecular Immunology. 3:235-40. Ford JW, et al. 2009. Curr Opin Immunol. 21:38-46. Leitner J, et al. 2009. Eur J Immunol. 2009. 39:1754-64.
Gene ID: 80381
UniProt: View information about CD276 on UniProt.org
Clone: A20079E
Regulatory Status: RUO
Other Names: Cluster of differentiation 276 (CD276), B7 Homolog 3 (B7-H3), 4Ig-B7-H3, B7RP-2
Isotype: Mouse IgG1, κ