Biolegend, 617252, Purified anti-human Granzyme A Antibody, 100μg

Granzyme A is a serine protease belonging to the granzyme family and is expressed exclusively by cytotoxic T cells (CTL) and NK cells. Most circulating CD56+CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzymes A and B. In contrast, few circulating CD4+ T cells express granzyme A or B. Activation of CD8+ and CD4+ T lymphocytes induces substantial expression of granzyme B, but not granzyme A. Following receptor-mediated conjugate formation between a granzyme-containing cell and an infected or transformed target cell, granzymes enter the target cell via endocytosis and induce apoptosis. Granzyme A was found to induce caspase-independent cell death when it enters into the target cell by perforin. Once in a cell, granzyme A activates DNA nicking by DNAse NM23-H1, a tumor suppressor gene product whose expression is reduced in transformed, metastatic cells. Dysregulation of this pathway results in several human diseases, such as hemophagocytic lymphohistiocytosis. Besides the protease activity, granzyme A induces human lung fibroblasts to produce IL-6 and IL-8. Cytokine induction is abrogated by treating the serine protease with the suicide serine protease inhibitor 3,4-dichloroisocoumarin. Other fibroblast lines, as well as epithelial cells, produce cytokines in response to granzyme A. These findings suggest that granzyme A can function as an activation molecule with potentially important immunoregulatory functions. However, CTLs from mice lacking granzyme A induce morphologically normal apoptosis in vitro , but those from mice that are deficient of granzyme B induce the nuclear features of apoptosis (particularly DNA fragmentation) more slowly than do wild-type CTLs.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant Granzyme A Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W19350D) could function as a detection antibody when paired with purified anti-human Granzyme A antibody (Cat No. 617152) (clone W19350A) as the capture antibody and using Recombinant Human Granzyme A protein (Cat. Nos. 550802 and 550804) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3097179 (BioLegend Cat. No. 617252)
Structure: Monomer
Distribution: CTL and NK cells
Function: Granzyme A is able to induce target cell apoptosis by activating caspase independent pathways.
Interaction: Targets of CTL and NK cells
Cell Type: Tregs
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience
Molecular Family: Enzymes and Regulators, Proteases
Antigen References: Edwards KM, et al. 1999. J Biol Chem. 274:30468-73. Lieberman J and Fan Z. 2003. Curr Opin Immunol. 15:553-9. Fan Z, et al. 2003. Cell. 112:659-72. Sower LE, et al. 1996. Cell Immunol. 171:159-63. Grossman WJ. et al. 2004. Blood. 104:2840-8. Ebnet K, et al. 1995. EMBO J. 14:4230-9. Heusel JW, et al. 1994. Cell. 76:977-87.
Gene ID: 3001
UniProt: View information about Granzyme A on UniProt.org
Clone: W19350D
Regulatory Status: RUO
Other Names: Hanukah factor serine protease (HFSP), Cytolytic T cell-and natural killer cellspecific trypsin-like serine protease, Cytotoxic T-lymphocyte-associated serineesterase 3 (CTLA3)
Isotype: Mouse IgG2b, κ