Biolegend, 618052, Purified anti-human IL-22 Antibody, 100μg

IL-22 is a cytokine structurally related to IL-10. It was originally identified in mouse as a gene induced by IL-9 in T cells and mast cells. IL-22 was initially designated as ILTIF (IL-10-related T cell-derived inducible factor). IL-22 belongs to a family of cytokines with limited homology to IL-10, namely IL-10, IL-19, IL20, IL-24, IL-26, IL-28A, IL-28B and IL-29 (the later three also known as IFN-λ). Human IL-22 shares 79% amino acid identity with mouse IL-22 and 25% identity with human IL-10. IL-22 biological activity is initiated by the binding to a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein, IL-22BP, which share sequence similarity with an extracellular region of IL-22R1 (sIL-22R1). Both chains of the IL-22R complex belong to the class II CRF. Two types of IL-22 binding receptors have been discovered, a membrane-bound receptor and a soluble receptor, both encoded by different genes. IL-22 is produced by immune cells and acts on nonimmune cells to regulate local tissue inflammation. As a product of T helper 17 lineage of CD4 (+) effector lymphocytes, IL-22 plays a critical role in mucosal immunity as well as in deregulated inflammation observed in autoimmune diseases.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant IL-22 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone W20080F) could function as a detection antibody when paired with purified anti-human IL-22 antibody (Cat No. 617852) (clone A20056F) as the capture antibody using Recombinant Human IL-22 protein (Cat Nos. 571302, 571304, 571306, and 571308) as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3083220 (BioLegend Cat. No. 618052)
Structure: Cytokine
Distribution: Thymic lymphomas, T cells, activated T cells, macrophages, and mast cells
Function: Enhances the production of acute phase proteins in a hepatocyte cell line, HepG2; promotes Th17 cells to transmigrate through the blood-brain barrier in multiple sclerosis
Interaction: Interacts with a cell surface complex composed of IL-22R1 and IL-10R2 receptor chains which are expressed in epithelial cells of inflammatory diseases such as psoriasis, Crohn's disease, rheumatoid arthritis, or allergic asthma
Ligand/Receptor: IL-22R1 and IL-10R2 receptor chains
Biology Area: Cell Biology, Immunology, Stem Cells
Molecular Family: Cytokines/Chemokines
Antigen References: Nagalakshmi ML, et al. 2004. Intern Immunopharmacol 4:679-91. Kebir H, et al. 2007. Nat Med. 13:1173-5. Gu Y, et al. 2008. Eur J Immunol. 38:1807-13. Pene J, et al. 2008. J Immunol. 180:7423-30. Dumotier L, et al. 2000. J immune. 164:1814-1819. Xie MH, et al. 2000. J Biol Chem. 765:31335-9. Jones BC, et al. 2008. Structure. 1333-44. Kotenko SV, et al. J Immunol. 166:7096-103. Chang C, et al. 2006. Cell Res 16:902-7.
Gene ID: 50616
UniProt: View information about IL-22 on UniProt.org
Clone: W20080F
Regulatory Status: RUO
Other Names: IL22, IL-D110, IL-TIF, MGC79382, TIFIL-23, TIFa, zcyto18
Isotype: Mouse IgG2b, κ