Biolegend, 619252, Purified anti-Hippocalcin Antibody, 100μg

Hippocalcin, a member of the neuronal calcium (Ca 2+ ) sensor family, is a Ca 2+ -binding protein that translocates between membrane and cytosol through its Ca 2+ /myristoyl switch. Its structural composition consists of 191 amino acids, forming a compact protein with a molecular weight of approximately 21 kD. The protein is characterized by the presence of four EF-hand motifs, each containing a helix-loop-helix structure, facilitating high-affinity Ca 2+ binding. Through its Ca 2+ -sensing capabilities, hippocalcin modulates various crucial cellular functions and signaling pathways. It participates in the regulation of intracellular Ca 2+ homeostasis and influences Ca 2+ -dependent signaling cascades. The protein's interactions with key proteins and enzymes enables it to contribute to processes such as neurotransmitter release, synaptic plasticity, and neuronal excitability. Hippocalcin’s involvement in human diseases has been observed in several conditions. In neurological disorders like epilepsy, dysregulation of hippocalcin disrupts Ca 2+ signaling, leading to neuronal hyperexcitability and the manifestation of seizures. Additionally, alterations in hippocalcin expression and function have been linked to neurodegenerative diseases, including Alzheimer's and Parkinson's, suggesting its potential role in disease progression. Furthermore, elevated levels of hippocalcin have been associated with certain cancers, such as breast and lung cancer, indicating its involvement in tumor progression and metastasis.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of Hippocalcin
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product is not suitable for immunocytochemistry (ICC) and immunohistochemistry (IHC-P).
RRID: AB_3068101 (BioLegend Cat. No. 619251) AB_3068101 (BioLegend Cat. No. 619252)
Structure: Ca2+ -dependent oligomerization
Distribution: Abundant in brain and hippocampus; membrane/cytoplasmic translocation
Function: Bind to Ca2+ to regulate neuronal activity
Interaction: β2-adaptin subunit of the AP2 adaptor complex, PSD95
Ligand/Receptor: Ca2+
Cell Type: Mature Neurons, Neural Stem Cells, Neurons
Biology Area: Neuroscience, Signal Transduction
Molecular Family: Postsynaptic proteins, Synaptic Vesicle Trafficking/Endocytosis
Antigen References: O'Callaghan DW, et al. 2003. J Cell Biol. 163:715-21. Palmer CL, et al. 2005. Neuron. 47:487-94. Kobayashi M, et al. 2005. Neuroscience. 133:471-84. Kerrigan TL, et al. 2017. Front Mol Neurosci. 5:57. Park S-Y, et al. 2017. Stem Cell Reports. 8:95-111.
Gene ID: 3208
UniProt: View information about Hippocalcin on UniProt.org
Clone: A18228E
Regulatory Status: RUO
Other Names: HPCA, Neuron-specific calcium-binding protein hippocalcin, Calcium-Binding Protein BDR-2, BDR-2
Isotype: Mouse IgG2b, κ