Biolegend, 620102, Purified anti-ZAP70 Phospho (Tyr493) Antibody, 100μg

ZAP70 is a member of the Syk protein tyrosine kinase subfamily that is expressed exclusively in T cells and NK cells that plays an essential role in T cell receptor (TCR) signaling in combination with the Src family kinases, LCK and FYN. Upon TCR stimulation, phosphorylation of CD3 by LCK leads to the recruitment of ZAP70 to the activated receptor. Upon association of ZAP70 with the activated TCR complex, the kinase is phosphorylated on several tyrosine residues, including Tyrosine 493. Phosphorylation of Tyrosine 493, which is found within the activation loop of the protein, results in increased enzymatic activity. ZAP70 subsequently phosphorylates downstream targets, including the adaptor molecules LAT and LCP2, facilitating the recruitment and activation of other signaling molecules, ultimately leading to T cell proliferation, differentiation, and activation. ZAP70 mays also serve as a scaffold for recruiting additional factors to the activated TCR, and can function to regulate TCR-CD247/CD3Z expression at the plasma membrane. ZAP70 deficiency results in severely impaired immune function, while higher levels of ZAP70 expression are associated with certain B cell malignancies. PTN22 is required for the dephosphorylation of ZAP70 Tyrosine 493.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human ZAP70 peptide phosphorylated at Tyr493
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 ug/mL (1:1000-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_2716044 (BioLegend Cat. No. 620101) AB_2716045 (BioLegend Cat. No. 620102)
Structure: 619 amino acid protein with a predicted molecular weight of 70 kD. Contains 2 N-terminal SH2 domains and a C-terminal kinase domain.
Distribution: T cells and NK cells
Function: Protein tyrosine kinase, T cell signaling
Interaction: CD247/CD3Z, NFAM1, SLA, , FCRL3, VAV1, CBL, RHOH, DEF6
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Katz ZB, et al. 2017. Nat Immunol.18:86. Klammt C, et al. 2015. Nat Immunol. 16:961. Jenkins MR, et al. 2014. eLife. 3:e01310 Boldizsar F, et al. 2013. Mol Immunol. 53:111. Wu J, et al. 2006. J. Biol Chem. 281:11002. Kong G, et al. 1996. Mol. Cell. Biol. 16:5026. Wange RL, et al. 1995. J. Biol. Chem. 270:18730. Chan AC, et al. 1995. EMBO. J.14:2499.
Gene ID: 7535
UniProt: View information about ZAP70 Phospho Tyr493 on UniProt.org
Clone: A16043C
Regulatory Status: RUO
Other Names: SRK, STD, TZK, p Zeta-Chain (TCR) Associated Protein Kinase 70 kD, p 70 KD Zeta-Chain Associated Protein, p Tyrosine-protein kinase ZAP-70
Isotype: Mouse IgG1, κ