Biolegend, 620453, Ultra-LEAF™ Purified anti-human Noggin Antibody, 100μg

Noggin (NOG) is an embryonic inducer that can sequester TGF-β cytokines of the BMP family with extremely high affinity. This protein was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. NOG also has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. It is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle, and skin. During culture of human embryonic stem cells (human ESC) or neural stem cells under certain conditions, addition of NOG to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons. NOG also appears to maintain adult stem cell populations in vivo . For example, it maintains neural stem cells within the hippocampus. Targeted deletion of NOG in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system. Conversely, transgenic mice over-expressing NOG in mature osteoblasts display impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human Noggin
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing alkaline phosphatase production of ATDC5 mouse chondrogenic cells which was inhibited by recombinant human Noggin (Cat. No. 795904) at 0.5 µg/mL and human BMP-4 (Cat. No. 795604) at 50 ng/ml. ND50 range: 0.3-2 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3083405 (BioLegend Cat. No. 620453) AB_3083405 (BioLegend Cat. No. 620454)
Structure: Cytokine
Distribution: Skeletal muscles, lungs, neurons, and skin
Function: Antagonizes BMPs
Interaction: Mesenchymal stem cells
Ligand/Receptor: BMPs
Cell Type: Embryonic Stem Cells, Mesenchymal Stem Cells
Biology Area: Cell Biology, Immunology, Neuroscience, Signal Transduction, Stem Cells, Synaptic Biology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Valenzuela DM, et al. 1995. J Neurosci. 15:6077-84. McMahon JA, et al. 1998. Genes Dev. 12:1438-52. Yuasa S, et al. 2005. Nat Biotechnol. 23:607-11. Gong Y, et al. 1999. Nat Genet. 21:302-4. Chiba S, et al. 2008. Stem Cells. 26:2810-20. Bonaguidi MA, et al. 2008. J Neurosci. 28:9194-204.
Gene ID: 9241
UniProt: View information about Noggin on UniProt.org
Clone: A15144A
Regulatory Status: RUO
Other Names: SYM1, SYNS1, NOG, Symphalangism 1, SYNS1A
Isotype: Mouse IgG2b, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.