Biolegend, 621602, Purified anti-human CD279 (PD-1) Antibody, 100μg

Programmed cell death protein 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC, CD273) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD279 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Direct ELISA - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. For Direct ELISA, the optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: A17188B antibody can block the binding of NAT105 and EH12.2H7 antibodies to the target.
Product Citations: Le X, et al. 2021. J Thorac Oncol. 16:583. PubMed
RRID: AB_2820103 (BioLegend Cat. No. 621602)
Structure: 55 kD type I transmembrane protein
Distribution: Transiently expressed on CD4- and CD8- thymocytes, upregulated in thymocytes and splenic T and B lymphocytes, and is expressed on activated myeloid cells.
Function: Signaling and co-stimulation (co-inhibition)
Interaction: SHP-1 and SHP-2
Ligand/Receptor: PD-L1 (CD274) and PD-L2 (CD273)
Cell Type: Lymphocytes, T cells
Biology Area: Cell Biology, Immuno-Oncology, Inhibitory Molecules
Molecular Family: CD Molecules
Antigen References: Ishida Y, et al. 1992. EMBO J. 11:3887 Francisco LM, et al. 2010. Immunol Rev. 236:219
Gene ID: 5133
UniProt: View information about CD279 on UniProt.org
Clone: A17188B
Regulatory Status: RUO
Other Names: PD1, PDCD1
Isotype: Mouse IgG2b, κ