Biolegend, 622403, Purified anti-p38 MAPK Antibody, 50microl

p38 mitogen-activated protein kinase (MAPK) is a member of the stress activated kinase family with a molecular weight of 38 kD. MAPK can be activated by a number of stimuli including heat, changes in osmolarity, irradiation, and cold. The MAPKs require dual phosphorylation (threonine and tyrosine) for enzymatic activation. The p38 MAP kinase is required for G2 cell cycle delay after UV irradiation, also participates in cytokine production (IL-6 and erythropoietin). The Poly6224 antibody recognizes human, mouse, and rat p38 MAPK and has been shown to be useful for Western blotting.
50microl
Verified Reactivity: Mouse, Rat, Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: peptide-KLH, C terminal
Formulation: PBS containing 0.09% sodium azide and 0.2% gelatin.Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:200 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product may contain other non-IgG subtypes.
Application References(PubMed link indicates BioLegend citation): da Silva, et al. 2011. Biochim Biophys Acta. 181:1863. PubMed Chen W, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:279. PubMed Maeda S, et al. 2014. J Biol Chem. 289:23786. PubMed
Product Citations: Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed
RRID: AB_2876740 (BioLegend Cat. No. 622403)
Structure: Belongs to serine/threonine kinase family, MAP subfamily, 38 kD
Distribution: Ubiquitously expressed, nuclear and cytoplasmic
Function: p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock-out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2, MEF2C and MAX.
Interaction: Interacts with MAX, Cdc25B, Cdc25C. Binds to kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm.
Modification: Phosphorylation
Biology Area: Cell Biology
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Bulavin DV, et al. 2001. Nature 411:102. 2. Han J, et al. 1994. Science 265:808. 3. Lee JC, et al. 1994. Nature 372:739.
Regulation: Activated by environmental stress, many pro-inflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK.
Gene ID: 80824
UniProt: View information about p38 MAPK on UniProt.org
Clone: Poly6224
Regulatory Status: RUO
Other Names: Stress activated kinase p38, p38 MAP kinase, stress activated protein kinase 2A
Isotype: Rabbit Polyclonal IgG