Biolegend, 623701, Purified anti-TOP2A Antibody, 25μg

DNA Topoisomerase-II α, or TOP2A, alters the toplogical states of DNA during various cellular processes, including transcription, meiosis, mitosis, and chromatin segregation. The enzyme functions by breaking and religating two strands of DNA, allowing each strand to pass through one another. Multiple classes of anti-cancer drugs initiate cell death by inceasing the frequency of potentially deleterious TOP2A-mediated DNA breaks.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant protein corresponding to amino acids 1232-1531 of TOP2A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: W18080B failed IP testing.When used for ICC, W18080B may produce light cytosolic staining. It is unclear if this represents background or TOP2A localized to the cytosol (Ramamoorthy M, et al. 2012. Nucleic Acids Res. 40:1621).W18080B was ICC-tested on PFA-fixed HeLa cells permeabilized with Triton X-100 or methanol. The clone was compatible with both permeabilization methods.
RRID: AB_2814484 (BioLegend Cat. No. 623701) AB_2814485 (BioLegend Cat. No. 623702)
Structure: TOP2A is a 1,531 amino acid protein with a predicted molecular weight of 174 kD
Distribution: Nucleus/ubiquitously expressed
Function: Regulate DNA topology
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics, DNA Repair/Replication
Molecular Family: Enzymes and Regulators
Antigen References: Champoux JJ. 1977. Proc. Natl. Acad. Sci. USA. 74:5328. Brown PO and Cozzarelli NR. 1979. Science. 206:1081.
Gene ID: 7153
UniProt: View information about TOP2A on UniProt.org
Clone: W18080B
Regulatory Status: RUO
Other Names: DNA Topoisomerase II α, TOP2, DNA Gyrase
Isotype: Rat IgG2a, κ