Biolegend, 624502, Purified anti-STAT2 Antibody, 200microl

STAT2, a 113 kD member of the STAT (signal transducer and activators of transcription) protein family, is phosphorylated in response to interferon alpha receptor signaling by Janus kinase I. Phosphorylation of STAT 2 induces nuclear translocation to activate transcription. STAT2 is highly expressed in the skin and thymus. STAT 2 forms complex with STAT1 and p48 to activate transcription and does not bind DNA directly. STAT 3 interacts with interferon regulatory factor 9, interferon alpha receptor, STAT 1, p48, and CREB binding protein. The Poly6245 antibody recognizes the C-terminal region of human STAT2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
200microl
Verified Reactivity: Human
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Peptide mapping to the carboxy terminus of human STAT2
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
Preparation: The antibody was purified by antigen-affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.
Application: WB - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation. This product may contain other non-IgG subtypes.
Additional Product Notes: The 200 µL size can be used for approximately 20 Western blots.
RRID: AB_2198452 (BioLegend Cat. No. 624502)
Structure: STAT family, contains coiled coil and SH2 domain, 113 kD
Distribution: Translocates into nucleus after phosphorylation. Expressed in skin, thymus.
Function: Phosphorylated in response to cytokine signaling by receptor-associated kinases, translocates to nucleus to act as transcriptional activator. Interferon alpha binding to its receptor induces a transcription complex (ISGF3) composed of p48, STAT2, and STAT
Interaction: STAT1, p48, interferon regulatory factor 9, interferon receptor 2, CREB binding protein
Modification: Phosphorylation (Tyr 690) by Janus kinase 1
Biology Area: Cell Biology, Immunology, Signal Transduction, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Bluyssen HAR, et al. 1997. J. Biol. Chem. 272:4600. 2. Qureshi AA, et al. 1996. Mol. Cell. Biol. 16:288.
Regulation: Phosphorylated by signaling from IFN alpha receptor
Gene ID: 6773
UniProt: View information about STAT2 on UniProt.org
Clone: Poly6245
Regulatory Status: RUO
Other Names: Signal transducer and activator of transcription 2, interferon alpha induced transcriptional activator
Isotype: Rabbit Polyclonal IgG