Biolegend, 624851, Purified anti-SMAD2 Antibody, 25μg

SMAD2 is a potent regulator of different cellular processes such as apoptosis, differentiation, and cell proliferation. SMAD2 is associated with SARA protein and the TGF-β receptor. SMAD2 gets phosphorylated by the TGF-β receptor in response to TGF-β signaling, which induces its dissociation from SARA and association with SMAD4 followed by translocation into the nucleus. In the nucleus, the SMAD2/SMAD4 protein complex participates in gene regulation by forming a transcription repressor complex in association with other cofactors.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human SMAD2 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.016 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone cross-reacts with mouse. For intracellular flow cytometric staining with this clone, we recommended using Fixation Buffer (Cat. No. 420801), True-Phos™ Perm Buffer (Cat. No. 425401), Intracellular Staining Permeabilization Wash Buffer (Cat. No. 421002) or Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not applicable. We do not recommend this product for use in immunocytochemistry (ICC).
RRID: AB_3097247 (BioLegend Cat. No. 624851) AB_3097247 (BioLegend Cat. No. 624852)
Structure: SMAD2 is a 467 amino acid protein with a predicted molecular weight of 52 kD
Distribution: Skeletal muscle, endothelial cells, heart and placenta; non-phosphorylated SMAD2 is present in the cellular cytoplasm close to the TGF-β receptor; phosphorylated SMAD2 is present in the cellular nucleus and binds to target promoter sequences.
Function: Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Interaction: TAZ/WWRT1, FOXH1, SNW1, CREB-binding protein (CBP), EP300, SNON, ALK4/ACVR1B, SKOR1, SKOR2, PRDM16, LEMD3, RBPMS, WWP1, PDPK1, DAB2, USP15, PPP5C, LDLRAD4, PMEPA1, ZFHX3, ZNF451, SMURF2, PPM1A, TGF-β, TGFBR1, TGIF, SMAD3, TRIM33, ZNF580, NEDD4, HGS, AIP1, WWP1, PML, ZNF8, RNF111, YAP1
Ligand/Receptor: SMAD2 is part of the TGF-β signaling pathway but it is not a ligand/receptor protein
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Cycle/DNA Replication, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins
Antigen References: Nakao A, et al. 1997. EMBO J. 16:5353–62. Attisano L. and Wrana JL, et al. 2002. Science. 296:1646-47. Moustakas A. et al. 2001. J Cell Sci. 114: 4359-69.
Gene ID: 4087
UniProt: View information about SMAD2 on UniProt.org
Clone: W21150C
Regulatory Status: RUO
Other Names: MAD homolog 2, JV18-1, MAD-2, MADH2, MADR2, Mad-related protein 2, Mothers against DPP homolog 2
Isotype: Rat IgG2b, κ