Biolegend, 625151, Purified anti-CD2AP Antibody, 25μg

CD2‐associated protein (CD2AP) is a scaffolding molecule connecting membrane proteins to the cytoskeleton. CD2AP also participates in the immunological synapse between CD2-expressing T cells and antigen-presenting cells. T cell activation induces cell adhesion through CD2-mediated binding of antigen-presenting cells to surface ligands. This enhances antigen-specific T cell activation, cell clustering, and induces cytoskeletal polarization. The interactions between CD2AP and other cytoskeletal proteins can regulate EGFR endocytosis. CD2AP is expressed at highest levels in liver, thymus, and spleen. Ablation of CD2AP disrupted the tissue architecture of the lamina propria cells of the gastric mucosa, indicating that CD2AP plays an important role in maintaining the normal morphology of the gastric mucosa. CD2AP-deficient mice develop a fatal congenital nephrotic syndrome, suggesting that CD2AP is also involved in maintaining renal glomerular integrity.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant human CD2AP protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone does not cross-react with mouse in western blotting (WB). For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended. For immunohistochemistry (IHC-P), antigen retrieval buffer Citrate Buffer (Cat. No. 420901) or Tris-EDTA pH 9.0 is recommended.
Additional Product Notes: This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.
RRID: AB_3097249 (BioLegend Cat. No. 625151) AB_3097249 (BioLegend Cat. No. 625152)
Structure: CD2AP is a 639 amino acid protein with a predicted molecular weight of 71 KD
Distribution: Widely expressed in fetal and adult tissues
Function: Regulating signal transduction and cytoskeletal molecules
Interaction: F-actin, PKD2, NPHS1, NPHS2, WTIP, DDN, CBL, BCAR1/p130Cas, MVB12A, ARHGAP17, ANLN, CD2, CBLB, PDCD6IP, TSG101, RIN3, CGNL1 and SH3BP1
Cell Type: Neurons, T cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience
Molecular Family: Adaptor Proteins, APP/β-Amyloid
Antigen References: Kirsch KH, et al. 1999. EMBO J. 96:6211-16. Kirsch KH, et al. 2001. J Biol Chem. 276:4957-63. Lynch DK, et al. 2003. J. Biol. Chem. 278:21805-13. Hutchings NJ, et al. 2003. J. Biol. Chem. 278:22396-403. Shih NY. et al. 1999. Science. 286:312-15.
Gene ID: 23607
UniProt: View information about CD2AP on UniProt.org
Clone: W21094A
Regulatory Status: RUO
Other Names: Adapter protein CMS, AL024079, C78928, Cas ligand with multiple SH3 domains, CD2 associated protein, CMS, Mesenchyme to epithelium transition protein with SH3 domains 1, METS 1
Isotype: Rat IgG2a, κ