Biolegend, 625852, Purified anti-ATG7 Antibody, 100μg

ATG7 is an E1-like ligase that plays a central role in autophagosome biogenesis by conjugating ATG5 to ATG12 which degrades and recycles damaged organelles, protein aggregates, and invading pathogens. ATG7 interacts with other ATG proteins, such as ATG3, ATG10, and ATG16L1, to form a complex that is required for autophagy, also regulates immunity, cell death, and protein secretion, and independently regulates the cell cycle and apoptosis. It also participates in lipidation of the ATG8 protein, which is essential for autophagosome formation. Additionally, ATG7 has been shown to interact with other proteins outside of the autophagy pathway, such as p53, to regulate apoptosis. Disrupting ATG7-dependent autophagy has been shown to cause electromotility disturbances, outer hair cell loss, and deafness in mice. ATG7 dysfunction has been associated with various human diseases, including cancer, neurodegeneration, and infection. ATG7 dysfunction and disease was found in patients with biallelic ATG7 variants and childhood-onset neuropathology. Furthermore, ATG7 has been shown to induce basal autophagy and rescue autophagic deficiency in CryAB R120G cardiomyocytes. This product was carefully developed for performance and specificity using the following products from Horizon Discovery, a Revvity company: HAP1 ATG KO cells (Horizon Cat. No. HZGHC000302c022)
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human ATG7
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P, KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 5.0 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For immunohistochemistry (IHC-P), Citrate Buffer, 10X (Cat. No, 420902) is recommended. For immunocytochemistry (ICC), either Fixation Buffer (Cat. No. 420801) or paraformaldehyde-based fixation is recommended. Clone A17129A detects homodimer of ATG7 in western blotting (WB). The homodimer band is demolished by increasing DTT concentration in the lysis buffer.
RRID: AB_3083415 (BioLegend Cat. No. 625851) AB_3083415 (BioLegend Cat. No. 625852)
Structure: Homodimer
Distribution: Cytosol and preautophagosomal structure
Function: Conjugate ATG12 to ATG5, lipidation of ATG8
Interaction: Interacts with ATG3, ATG8, ATG12, EP300 and FOXO1
Cell Type: Mature Neurons, Neurons
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Cell Proliferation and Viability, Neurodegeneration, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Ubiquitin/Protein Degradation
Molecular Family: Autophagosome Markers, Ligases
Antigen References: Komatsu M, et al. 2005. J Cell Biol. 169:425-34. Komatsu M, et al. 2007. Proc Natl Acad Sci USA. 104:14489-94. Lee I H, et al. 2012. Science. 336: 225–228. Collier JJ, et al. 2021. EMBO Mol Med. 13:e14824.
Gene ID: 10533
UniProt: View information about ATG7 on UniProt.org
Clone: A17129A
Regulatory Status: RUO
Other Names: APG7, APG7-like, APG7L, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-Like Modifier-Activating Enzyme ATG7, Autophagy-related protein 7
Isotype: Mouse IgG2b, κ