Biolegend, 626752, Purified anti-NOS3 (eNOS) Antibody, 100μg

Nitric oxide synthase 3 (NOS3), also commonly known as endothelial nitric oxide synthase (eNOS), is a critical enzyme primarily found in the endothelial cells lining the blood vessels. Its main function is to catalyze the formation of nitric oxide (NO) from L-arginine. NO is a signaling molecule with profound effects on vascular health and overall physiology, regulating blood vessel tone and diameter in response to various physiological cues including shear stress, neurotransmitters, and hormones. NO relaxes and dilates blood vessels, thereby promoting proper blood flow and reducing blood pressure. Additionally, NOS3-derived NO helps prevent the formation of blood clots, reduces inflammation in the blood vessel walls, and maintains vascular homeostasis, contributing significantly to cardiovascular health. Dysfunction or impaired activity of NOS3 is implicated in various cardiovascular diseases, making it an important target for therapeutic interventions aimed at preserving vascular function.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant KLH-conjugated peptide fragment of human NOS3
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: IHC-P - Quality tested ICC, WB, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody clone, W22110B, does not cross-react with mouse NOS3 in Western Blot application. For immunohistochemistry (IHC-P), either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 is recommended for antigen retrieval system. For immunocytochemistry (ICC), Fixation Buffer (Cat. No. 420801) or 4% paraformaldehyde fixation followed by permeabilization with 0.5% Triton X-100 is recommended. Fixation/permeabilization with methanol is not recommended.
RRID: AB_3097356 (BioLegend Cat. No. 626751) AB_3097356 (BioLegend Cat. No. 626752)
Structure: NOS3 is a 1,205 amino acid protein with a predicted molecular weight of 133.3 kD.
Distribution: Cytoplasmic, vascular endothelial cells
Function: Enzyme, nitric oxide (NO) synthesis from L-arginine. Transcriptionally regulated by SP1, SP3, ETS-1, YY1.
Interaction: NOS3 is regulated via binding of its cofactor BH4, which is required to efficiently produce NO
Cell Type: Endothelial cells
Biology Area: Cardiovascular Biology
Molecular Family: Enzymes and Regulators
Antigen References: Mineo C and Shaul PW. 2012. Adv Exp Med Biol. 729:51-62. Hong FF, et al. 2019. Inflamm Res. 68:429-441. Siragusa M and Fleming I. 2016. Pfulgers Arch. 468:1125-1137. Musicki B and Burnett AL 2006. Exp Bil Med. 231:154-65.
Gene ID: 4846
UniProt: View information about NOS3 on UniProt.org
Clone: W22110B
Regulatory Status: RUO
Other Names: Nitric oxide synthase 3 (NOS3), Endothelial Nos (eNos), constitutive NOS (cNOS)
Isotype: Rat IgG2b, κ