Biolegend, 627101, Purified anti-NTAL Antibody, 25μg

Non-T cell activation Linker (NTAL) is a transmembrane adaptor protein with an apparent molecular weight of 30 kD expressed on B cells, natural killer (NK) cells, and myeloid cells. The NTAL protein is found in rafts, which are membrane microdomains that participate in B cell activation. NTAL interacts with a variety of proteins including Grb2, PLC gamma 2, and PLC gamma 1. This gene is one of the contiguous genes at 7q11.23 commonly deleted in Williams syndrome.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant cytoplasmic domain (91-243 a.a.) of human NTAL.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µl/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.
Product Citations: Brühl H, et al. 2014. J Leukoc Biol. 65:96. PubMed
RRID: AB_2133251 (BioLegend Cat. No. 627102)
Structure: 30 kD double palmitoylated protein gene that consists of 14 exons, and its alternative splicing generates three transcript variants.
Distribution: B, natural killer cells, monocytes, mast cells, and activated T cells.
Function: Involved in FCER1 mediated signaling in mast cells and as a negative regulator of early B cell signaling. May also be involved in CFCGR1 mediated signaling in myeloid cells. Couples activation of these receptors with distal intracellular events through the recruitment of Grb2.
Ligand/Receptor: Membrane microdomains (rafts) of B cells, natural killer (NK) cells, and myeloid cells.
Cell Type: B cells, Mast cells, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Yamasaki S, et al. 2007. Mol. Cell Biol. 27(12):4406-15. 2. Brdicka T, et al. 2002. J. Exp. Med. 196(12):1617-26. 3. Tkaczyk C, et al. 2004. Blood 104(1):207-14. 4. Volna P, et al. 2004. 200(8):1001-13. 5. Lebduska P, et al. 2007. 328(1-2):139-51.
Gene ID: 7462
UniProt: View information about NTAL on UniProt.org
Clone: NAP-07
Regulatory Status: RUO
Other Names: LAT2 (linker for activation of T cells family), member 2, LAB
Isotype: Mouse IgG1, κ