Biolegend, 627201, Purified anti-TRIM Antibody, 25μg

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human TRIM (amino acids 29-186)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, ICFC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, ELISA1, and immunofluorescence microscopy2.Clone TRIM-4 has been shown to cross-react to mouse TRIM1.
Application References(PubMed link indicates BioLegend citation): Huynh T, et al. 2001. Scand. J. Immunol. 54:146. Kirchgessner H, et al. 2001. J. Exp. Med. 193:1269.
RRID: AB_2240982 (BioLegend Cat. No. 627202)
Structure: Type III membrane protein, 30 kD disulfide-linked dimeric phosphoprotein
Distribution: T cells, lower expression levels in NK cells
Function: Links extracellular and intracellular signaling pathways in T cell activation
Interaction: PI3K regulatory subunit α, TCRα, β, and ζ.
Modification: Tyrosine phosphorylation by Src kinases
Cell Type: NK cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Bruyns E, et al. 1998. J. Exp. Med. 188:561. 2. Hubener C, et al. 2000. Immunogenetics 51:154.
Gene ID: 50852
UniProt: View information about TRIM on UniProt.org
Clone: TRIM-4
Regulatory Status: RUO
Other Names: T cell receptor-interacting molecule
Isotype: Mouse IgG2a, κ