Biolegend, 627204, PE anti-TRIM Antibody, 100tests

TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.
100tests
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Partial recombinant human TRIM (amino acids 29-186)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: intracellular flow cytometry1, ELISA1, and immunofluorescence microscopy2.Clone TRIM-4 has been shown to cross-react to mouse TRIM1.
Application References(PubMed link indicates BioLegend citation): Huynh T, et al. 2001. Scand. J. Immunol. 54:146.Kirchgessner H, et al. 2001. J. Exp. Med. 193:1269.
RRID: AB_2565761 (BioLegend Cat. No. 627203) AB_2565762 (BioLegend Cat. No. 627204)
Structure: Type III membrane protein, 30 kD disulfide-linked dimeric phosphoprotein
Distribution: T cells, lower expression levels in NK cells
Function: Links extracellular and intracellular signaling pathways in T cell activation
Interaction: PI3K regulatory subunit α, TCRα, β, and ζ.
Modification: Tyrosine phosphorylation by Src kinases
Cell Type: T cells, NK cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Antigen References: 1. Bruyns E, et al. 1998. J. Exp. Med. 188:561.2. Hubener C, et al. 2000. Immunogenetics 51:154.
Gene ID: 50852
UniProt: View information about TRIM on UniProt.org
Clone: TRIM-4
Regulatory Status: RUO
Other Names: T cell receptor-interacting molecule
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.