Biolegend, 627452, Purified anti-AKT Phospho (Ser473) Antibody, 200microl

AKT (also known as protein kinase B alpha) is a 60 kD serine/threonine-specific kinase containing a pleckstrin domain. AKT plays a critical role in controlling survival and apoptosis. This kinase is ubiquitously expressed and translocates to the membrane upon cell activation. It is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3-kinase. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473. The previously elusive PDK2, responsible for phosphorylation of AKT at Ser473, has been identified as a mammalian target of rapamycin (mTOR) in a rapamycin-insensitive complex with RICTOR and SIN1. Activated AKT phosphorylates a wide range of substrates including transcription factors (e.g. FOXO1), kinases (GSK-3beta, Raf-1, ASK, Chk1) and other proteins with important signaling roles (e.g. Bad, MDM2). AKT promotes cell survival by inhibiting apoptosis through phosphorylation of several targets, including Bad, forkhead transcription factor, c-Raf and caspase-9. AKT also plays a critical role in cell growth by directly phosphorylating mTOR in a rapamycin-sensitive complex containing raptor. More importantly, AKT phosphorylates and inactivates tuberin (TSC2), an inhibitor of mTOR within the mTOR-raptor complex.
200microl
Verified Reactivity: Human, Mouse
Antibody Type: Polyclonal
Host Species: Rabbit
Immunogen: Synthetic peptide of human AKT phosphorylated at Ser473
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested dilution of this reagent is 1:1000 - 1:4000. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This clone was tested by Western Blot using lysates prepared from HeLa cells untreated or treated with IGF-1, and NIH/3T3 cells untreated or treated with PDGF-BB.
RRID: AB_3097484 (BioLegend Cat. No. 627451) AB_3097484 (BioLegend Cat. No. 627452)
Structure: AKT is a 541 amino acid protein with a predicted molecular weight of 62 kD
Distribution: Ubiquitously expressed, translocates to the membrane upon activation
Function: Catalytically inactive multimeric complex. AKT is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473 by PDK2.
Interaction: Interacts through pleckstrin homology domain with second messengers. Interacts with AKTIP and CDKN1B.
Biology Area: Cell Adhesion, Cell Biology, Cell Death
Molecular Family: Phospho-Proteins, Protein Kinases/Phosphatase
Antigen References: Jacinto E, et al. 2006. Cell. 127:125-37. Inoki K, et al. 2002. Nat Cell Biol. 4:648-57. Franke TF, et al. 1995. Cell. 81:727-36. Brunet A, et al. 1999. Cell. 96:857-68. Yang WL, et al. 2010. Cell Cycle. 9:487-97.
Gene ID: 207
UniProt: View information about AKT on UniProt.org
Clone: Poly23A04
Regulatory Status: RUO
Other Names: Protein kinase B alpha, PRK-BA, PKB, Serine/Threonine specific kinase RAC alpha, Protein kinase Akt, RAC
Isotype: Rabbit Polyclonal IgG