Biolegend, 627801, Purified anti-Eg5 Antibody, 25μg

Eg5 (also known as kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, and thyroid receptor interacting protein 5 (TRIP5)) is a 119 kD kinesin-like protein family, BimC subfamily. This protein is a catalytic kinesin motor with coiled-coil and Smc domains. Eg5 is localized at the centrosomes, spindle microtubules, and intracellular bridge. This motor protein is required for establishing the bipolar spindle. hEg5 is modified by phosphorylation on Thr927 by Cdc2 to allow association with the spindle apparatus. Eg5 has been shown to interact with the thyroid hormone receptor in presence of thyroid hormone and Cdc2. The 10C7/Eg5 monoclonal antibody has been shown to react with human and mouse Eg5 by Western blot.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino Acid: 200-500 of human Eg5
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Chou HYE, et al. 2006. J. Biol. Chem. 281:15201. Irelan JT, et al. 2007. P. Natl. Acad. Sci. USA 104:16940. Bartoli, KM., et al. 2011. Mol Cell Biol. 22:3420. PubMed Izumiyama T, et al. 2012. Gene. 511:202. PubMed
Product Citations: Gury-BenAri M, et al. 2016. Cell. 1231:166. PubMed Bartoli K, et al. 2011. Mol Biol Cell. 3.291666667. PubMed Fang CT, et al. 2020. Cell Death Dis. 0.954861111. PubMed Izumiyama T, et al. 2012. Gene. 511:202. PubMed
RRID: AB_2130964 (BioLegend Cat. No. 627801) AB_2130963 (BioLegend Cat. No. 627802)
Structure: Kinesin-like protein family, BimC subfamily, kinesin motor catalytic, coiled-coil, Smc domains; 119 kD
Distribution: Centrosomes, spindle microtubules, intracellular bridge
Function: Motor protein required for establishing bipolar spindle
Interaction: Thyroid hormone receptor in presence of thyroid hormone, Cdc2
Modification: Phosphorylation
Biology Area: Cell Biology, Cell Cycle/DNA Replication
Antigen References: 1. Lee J, et al. 1995. Mol. Endocrinol. 9:243. 2. Blangy A, et al. 1995. Cell 83:1159. 3. Whitehead C, et al. 1998. J. Cell Sci. 111:2551. 4. DeBonis S, et al. 2003. Biochemistry. 42:338.
Regulation: Phosphorylation on Thr927 by Cdc2 allows association with spindle apparatus
Gene ID: 3832
UniProt: View information about Eg5 on UniProt.org
Clone: 10C7/Eg5
Regulatory Status: RUO
Other Names: Kinesin-like protein KIF11, kinesin-related motor protein Eg5, kinesin-like spindle protein HKSP, thyroid receptor interacting protein 5 (TRIP5)
Isotype: Mouse IgG1, κ