Biolegend, 628301, Purified anti-Lck Antibody, 25μg

Lck is a 56 kD tyrosine protein kinase and a member of the src subfamily that contains both an SH2 and SH3 domain. Alternatively spliced isoforms of Lck have been reported. Lck is a cytoplasmic protein bound to CD4 and CD8 in T lymphocytes that participates in antigen-induced T cell activation through phosphorylation of the T cell receptor zeta chain. Lck is activated upon T cell receptor engagement and is involved in the onset of cell cycle progression induced by interleukin-2. Phosphorylation by Csk downregulates the activity of Lck. In addition to CD4 and CD8, Lck has been reported to interact with PI3K through its SH3 domain and tyrosine phosphorylated KHDRBS1/p70 through its SH2 domain. In addition, Lck has been shown to associate with the SAP transmembrane tyrosine phosphatase. The Lck-01 monoclonal antibody recognizes human Lck and has been shown to be useful for Western blotting, immunoprecipitation, immunocytochemistry, and flow cytometry.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Peptide corresponding to a.a. 22-36 in human Lck
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified FC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/ml is recommended. For immunoprecipitation, the suggested use of this reagent is 2.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Does not cross-react with mouse. Clone may cross-react with other Src tyrosine kinase family members.
Application References(PubMed link indicates BioLegend citation): Romagnoli P, et al. 2001. Int. Immunol. 13:305.
Product Citations: Snodgrass RG, et al. 2018. Front Immunol. 1906:9. PubMed Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed Baumgart F, et al. 2016. Nat Methods. 10.1038/nmeth.3897. PubMed Wang Y, et al. 2021. Cell Reports. 36(6):109516. PubMed
RRID: AB_2136337 (BioLegend Cat. No. 628301) AB_2136336 (BioLegend Cat. No. 628302)
Structure: Belongs to the tyrosine family of kinases, src subfamily. Contains one SH2 domain, 1 SH3 domain. Approximately 56 kD, alternatively spliced isoforms have been reported
Distribution: Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes
Function: Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression
Interaction: Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase
Modification: Phosphorylation
Cell Type: T cells
Biology Area: Cell Biology, Signal Transduction
Molecular Family: Protein Kinases/Phosphatase
Antigen References: 1. Koga Y, et al. 1986. Eur. J. Immunol. 16:1643. 2. Vogel LB, et al. 1995. Biochem. Biophys. Acta 1264:168. 3. Vogel LB, et al. 1993. Mol. Cell. Biol. 13:7408. 4. Bergaman M, et al. 1992. EMBO J. 11:2919.
Regulation: Activated upon T cell receptor engagement. Tyrosine phosphorylation by p50 Csk downregulates catalytic activity
Gene ID: 3932
UniProt: View information about Lck on UniProt.org
Clone: LCK-01
Regulatory Status: RUO
Other Names: Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase
Isotype: Mouse IgG2a, κ