Biolegend, 628402, Purified anti-Cytokeratin 18 Antibody, 100μg

Cytokeratin 18, also known as keratin 18, is a type I intermediate filament protein with a molecular weight of approximately 48 kD. Cytokeratin 18 is a heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8 and is widely expressed in simple epithelial tissues in adults, where it is found in the cytoplasm, nucleus, and nucleolus. Cytokeratin 18 is associated with the cytoskeleton and mutations in this protein have been associated with liver disease after environmental insult. Cytokeratin 18 can be modified by glycosylation, phosphorylation (Ser34 and Ser53), acetylation (Ser2), and proteolytic cleavage at amino acids 238 and 397 by caspase family members. The cytokeratin 18 protein interacts with a number of proteins including caspase 3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, and usherin, among others.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human breast carcinoma cell line PMC-42
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - VerifiedELISA, IHC-P, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 µg per ml. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The DA-7 monoclonal antibody recognizes human cytokeratin 18 and is useful for Western blotting. Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, and ELISA.This clone and BioLegend clone LDK18 displayed similar affinities when tested for western blot applications
Application References(PubMed link indicates BioLegend citation): Taylor-Papadimitriou J, et al. 1989. J. Cell Sci. 94:403. Lauerova L, et al. 1988. Hybridoma 7:495.
Product Citations: Valentine H, et al. 2022. Cancer Res Commun. 2:1355. PubMed Urano-Takaoka M, et al. 2022. Acta Derm Venereol. 102:adv00636. PubMed
RRID: AB_439770 (BioLegend Cat. No. 628401) AB_439771 (BioLegend Cat. No. 628402)
Structure: Type I intermediate filament protein, contains three coiled-coil domains, approximately 48 kD. Heterotetramer composed of two type I and two type II keratins. Cytokeratin 18 associates with cytokeratin 8.
Distribution: Widely expressed in simple epithelial tissues in adults. Can be found in the cytoplasm, nucleus, and nucleolus
Function: Intermediate filament protein involved with the cytoskeleton. Mutations in cytokeratin 18 may predispose to liver disease upon exposure to some viruses or hepatoxins
Interaction: Interacts with a number of proteins including caspase3, 14-3-3 gamma, 14-3-3 zeta, 14-3-3 sigma 14-3-3 beta, 14-3-3 eta, keratin 5, keratin 8, TNF receptor II, plakophilin 2, EGF receptor, usherin
Modification: Phosphorylation (Ser34, Ser53), glycosylation, acetylation (Ser2). Can also be cleaved at amino acid 238 and 397 by caspase family members
Cell Type: Epithelial cells
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Ku N, et al. 2003. Proc. Natl. Acad. Sci. 100:6063. 2. Kulesh DA and Oshima RG. 1989. Genomics 4:339. 3. Steinart PM and Roop DR. 1988. Ann. Rev. Biochem. 57:593.
Gene ID: 3875
UniProt: View information about Cytokeratin 18 on UniProt.org
Clone: DA-7
Regulatory Status: RUO
Other Names: Keratin 18, Keratin type I cytoskeletal 18
Isotype: Mouse IgG1, κ