Biolegend, 628508, Alexa Fluor® 488 anti-Cytokeratin 19 Antibody, 100μg

Cytokeratin 19, also known as keratin 19, is a type I intermediate filament protein with a molecular weight of approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits. Unlike other cytokeratins, cytokeratin 19 lacks a C-terminal non-helical extension. This cytokeratin is widely expressed in the periderm (transient superficial layer enveloping developing epidermis), muscle, intestine, bile duct, esophagus, stomach, and thymus. Cytokeratin 19 can be upregulated by vitamin A and is thought to play a critical role in embryogenesis. Cytokeratin 19 intereacts with the pinin protein and has been shown to be modified by phosphorylation (Ser10, Ser35).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human mammary carcinoma cell line MCF-7
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.1 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, immunocytochemistry, and ELISA.
Application References(PubMed link indicates BioLegend citation): Karsten U, et al. 1985. Eur. J. Cancer Clin. Oncol. 21:733. Nishikata T,et al. Anticancer Res. 33:2867. PubMed
Product Citations: Richert LE, et al. 2014. Eur J Immunol. 397:44. PubMed
RRID: AB_2566632 (BioLegend Cat. No. 628508)
Structure: Type I intermediate filament protein, contains three coiled-coil domains, lacks C-terminal non-helical extension found in other keratins, approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits.
Distribution: Expressed in the periderm (transient superficial layer enveloping developing epidermis), also expressed in muscle, intestine, bile duct, esophagus, stomach, and thymus. Upregulated by vitamin A.
Function: Intermediate filament protein involved with the cytoskeleton. May play a critical role in embryogenesis
Interaction: Interacts with pinin
Modification: Phosphorylation (Ser10, Ser35)
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Antigen References: 1. Bader BL, et al. 1986. EMBO J. 5:1865. 2. Eckert RL. 1988. Proc. Natl. Acad. Sci. 85:1114. 3. Stasiak PC and Lane EB. 1987 Nucleic Acids Res. 15:10058.
Gene ID: 3880
UniProt: View information about Cytokeratin 19 on UniProt.org
Clone: A53-B/A2
Regulatory Status: RUO
Other Names: Keratin 19, Keratin 19 type 1 cytoskeletal
Isotype: Mouse IgG2a, κ