Biolegend, 628602, Purified anti-Cytokeratin (pan reactive) Antibody, 100μg

Cytokeratins are intermediate filament proteins that are widely expressed in many tissues. These proteins are vital components of the cytoskeleton and interact with a number of other proteins. Cytokeratins are usually found in the cytoplasm,although some cytokeratins can be found in the nucleus and the nucleolus.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Keratin enriched fraction from human epidermoid carcinoma.
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified IP, IHC-P - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 4.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The C-11 monoclonal antibody reacts with a conserved epitope of human cytokeratin 4, 5, 6, 8, 10, 13, and 18. This antibody has been shown to be useful for Western blotting and has also been reported to be useful for immunoprecipitation, immunohistochemistry (paraffin sections), immunocytochemistry, and spatial biology (IBEX)7,8.
Application References(PubMed link indicates BioLegend citation): Kovarik J. 1988. Int. J. Cancer Suppl. 3:50. Bartek J, et al. 1991. J. Pathol. 164:215. Chernyavsky A I, 2007. J. Biol. Chem. doi:10.1074/jbc.M611365200. PubMed Comito G, et al. 2012. Cancer Lett. 324:31. PubMed Vojt-òsek B, et al. 1989. Folia Biol (Praha). 35:373. (reactivity with rat, dog, sheep, pig, cow) Dekaney CM, et al. 2005. Gastroenterology 129:1567. (ICC) Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations: Yamashita S, et al. 2018. Oncol Lett. 15:400. PubMed Greier MDC, et al. 2023. Front Oncol. 13:1145817. PubMed Fang WB, et al. 2023. Front Oncol. 13:1130911. PubMed Nwosu ZC, et al. 2023. Nature. 618:151. PubMed Diray-Arce J, et al. 2023. Cell Rep Med. 4:101079. PubMed Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed Florian S, et al. 2019. Proc Natl Acad Sci U S A. 116:11444. PubMed Kesavan R, et al. 2021. Int J Mol Sci. 22:. PubMed Yao Y, et al. 2017. Cytometry B Clin Cytom. 92:12:00. PubMed Comito G, et al. 2012. Cancer Lett. 324:31. PubMed Baines KJ, et al. 2020. J Immunol. 204:694. PubMed Chernyavsky A, et al. 2007. J Biol Chem . 2.042361111. PubMed Bade RM, et al. 2021. Molecular Oncology. . PubMed Mayer J, et al. 2011. Cancer Genet. 204:589. PubMed Ran LJ, et al. 2018. Mol Med Rep. 18:723. PubMed Mukhtar E, et al. 2020. Cancer Drug Resist. 0.566666667. PubMed
RRID: AB_439774 (BioLegend Cat. No. 628601) AB_439775 (BioLegend Cat. No. 628602)
Structure: Intermediate filament protein, contains three coiled-coil domains. Cytokeratins exist as heterotetramers composed of two type I and two type II keratin subunits
Distribution: Cytokeratins are widely expressed proteins usually found in the cytoplasm although some cytokeratins can also be found in the nucleus and the nucleolus
Function: Intermediate filament protein involved with the cytoskeleton.
Interaction: Most cytokeratins interact with a number of other proteins such as 14-3-3, EGF receptor, and many others
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 3875
UniProt: View information about Cytokeratin pan reactive on UniProt.org
Clone: C-11
Regulatory Status: RUO
Other Names: Cytokeratin, Keratin
Isotype: Mouse IgG1, κ