Biolegend, 629001, Purified anti-PRC1 Antibody, 25μg

PRC1 (also known as protein regulator of cytokinesis 1 and mitotic spindle-associated CDK substrate) is a member of the MAP65/ASE1 family with a molecular weight of approximately 72 kD. PRC1 localization changes with cell-cycle position and can be found in the nucleus, the mitotic spindle, and the cell mid-body. PRC1 is a mitotic spindle-associated CDK substrate protein required for cytokinesis and maintenance of the spindle midzone. PRC1 can be modified by phosphorylation and has been shown to interact with Cdc2 and Cdk2. The 6G2 antibody recognizes the human PRC1 protein and has been shown to be useful for Western blotting.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Amino Acid: 1-150
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested KO/KD-WB - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 2.5 - 5.0 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Neahring L, et al. 2021. Dev Cell. 56:3006. PubMed Udy D, et al. 2015. PLoS One. 10: 0134738. PubMed Elting MW et al. 2017. Current biology : CB. 27(14):2112-2122 . PubMed Suresh P, et al. 2020. eLife. 9:e53807.. PubMed
RRID: AB_2169532 (BioLegend Cat. No. 629001) AB_2169531 (BioLegend Cat. No. 629002)
Structure: Human PRC1 has 4 isoforms with MW ranging from 61-72 kD.
Distribution: Cell cycle-dependent; nuclear, mitotic spindle, cell mid-body
Function: Mitotic spindle-associated CDK substrate protein required for cytokinesis, maintains the spindle midzone
Interaction: Cdc2, Cdk2
Modification: Phosphorylation
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Cell Motility/Cytoskeleton/Structure
Antigen References: 1. Jiang W, et al. 1998. Mol. Cell 2:877. 2. Mollinari C, et al. 2002. J. Cell Biol. 157:1175.
Gene ID: 9055
UniProt: View information about PRC1 on UniProt.org
Clone: 6G2
Regulatory Status: RUO
Other Names: Protein regulator of cytokinesis 1, Mitotic spindle-associated CDK substrate, Protein regulating cytokinesis 1
Isotype: Mouse IgG2b, κ