Biolegend, 629201, Purified anti-Tubulin-γ Antibody, 25μg

γ-tubulin is a 51 kD member of the tubulin superfamily and is conserved between yeast and mammals. This cytoskeletal protein is highly expressed in centrosomes and is thought to be involved in cytoskeletal organization and mitosis. γ-tubulin is expressed at highest levels during prophase to metaphase and can be modified by phosphorylation. This protein has been shown to interact with α-tubulin, paxillin, and RAC GTPase activated protein 1.
25μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Peptide-KLH
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality testedICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml (1:500-1:1000). For immunocytochemistry, a concentration of 2.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The purified 14C11 antibody has been shown to be useful for Western blotting of human, mouse, and rat ?-tubulin.
Product Citations: Amo L, et al. 2022. Front Immunol. 13:835527. PubMed Natalie Lerner, Sofia Avissar, Elie Beit-Yannai 2017. PLoS One. 12(2):e0171153. PubMed
RRID: AB_493419 (BioLegend Cat. No. 629201)
Structure: Tubulin superfamily, highly conserved between yeast and mammals. Predicted molecular weight approximately 51 kD
Distribution: Cytoskeletal protein, high expressed in centrosomes
Function: Cytoskeletal organization, mitosis
Interaction: Interacts with α-tubulin, paxillin, RAC GTPase activated protein 1
Modification: Phosphorylation
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family: Microtubules
Antigen References: 1. Zheng Y, et al. 1991. Cell 65:817.
Regulation: Expressed at highest levels during prophase to metaphase
Gene ID: 2776
UniProt: View information about Tubulin-gamma on UniProt.org
Clone: 14C11
Regulatory Status: RUO
Other Names: γ-tubulin
Isotype: Mouse IgG2b, κ