Biolegend, 629301, Purified anti-Elongin B Antibody, 25μg

Elongin B (also known as RNA polymerase II transcription factor SIII p18 subunit and transcription elongation factor B polypeptide 2) is a member of the UbH family containing a ubiquitin-like domain. Elongin functions as a subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. Clone 10C4 recognizes the human elongin B protein and is useful for Western blotting.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Full length recombinant protein
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality testedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations: Sato Y, et al. 2009. PLoS One. 5:e1000530. PubMed
RRID: AB_2201267 (BioLegend Cat. No. 629302)
Structure: UbH family, ubiquitin-like domain, heterotrimer; Human Elongin B has two isoforms with predicted MW of 13kD and 18kD
Distribution: Nuclear
Function: Regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites
Interaction: Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases
Biology Area: Cell Biology, DNA Repair/Replication, Transcription Factors, Ubiquitin/Protein Degradation
Antigen References: 1. Duan D, et al. 1995. Science. 269:1402. 2. Stebbins C, et al. 1999. Science. 284:455. 3. Brower C, et al. 2002. P. Natl. Acad. Sci. USA 99:10353. 4. DeRenzo C, et al. 2003. Nature. 424:685.
Gene ID: 6923
UniProt: View information about Elongin B on UniProt.org
Clone: 10C4
Regulatory Status: RUO
Other Names: RNA polymerase II transcription factor SIII p18 subunit, transcription elongation factor B polypeptide 2, TCEB2
Isotype: Mouse IgG1, κ