Biolegend, 629452, Purified anti-human Angiopoietin-2 Antibody, 100μg

Angiopoietin-2, also known as ANGPT2, is a member of angiopoietins, which plays essential roles in embryonic and postnatal angiogenesis. The structure of the mature form of ANGPT2 contains an N-terminal coiled-coil domain, which mediates multimerization of the protein, along with a C-terminal fibrinogen-like domain, which mediates the receptor binding. Both ANGPT1 and ANGPT2 are ligands for the receptor tyrosine kinase, Tie-2. ANGPT2 appears to exist predominantly as a homodimer, but it is also capable of forming multimers while ANGPT1 predominantly exists as multimers. It has been shown that ANGPT2 is able to promote vascular leakage and neutrophil migration. ANGPT2 can also sensitize TNF-α-induced monocyte adhesion, thus it is considered a proinflammatory factor. Although ANGPT2 is not required for arterial vascular development, its expression in the tissue suggests that it may play a role in the maintenance of these vessels. This conclusion is also supported by the observation that high concentrations of ANGPT2 activate the same survival signaling pathway as ANGPT1 to prevent endothelial cell apoptosis via Tie-2/Akt. Additionally, ANGPT2 was demonstrated to function as an agonist in the absence of ANGPT1, but in its presence it acts as an antagonist. Human ANGPT2 shares 86% amino acid sequence homology with its murine counterpart.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human Recombinant Angiopoietin-2 Protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: Direct ELISA - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone A15124B) could function as a detection antibody when paired with purified anti-human Angiopoietin-2 antibody (Cat No. 633752) (clone W19419E) as the capture antibody and using Recombinant Human Angiopoietin-2 (carrier-free) (Cat. Nos. 753102, 753104, and 753106) protein as the assay standard. For assay development flexibility purposes, this product is provided as a purified format.
RRID: AB_3717169 (BioLegend Cat. No. 629452)
Structure: Oligomer
Distribution: Placenta, uterus, ovaries, and vascular endothelial cells.
Function: ANGPT2 plays a role in angiogenesis and monocyte adhesion. Hyperoxia induces ANGPT2 expression in the lung epithelial cells.
Interaction: Monocytes and endothelial cells
Ligand/Receptor: Tie-2
Cell Type: Embryonic Stem Cells
Biology Area: Angiogenesis, Cell Biology, Stem Cells
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Augustin HG, et al. 2009. Nat Rev Mol Biol. 10:165-77. Ward NL and Dumont DJ. 2002. Semin Cell Dev Biol. 1:19-27. Roviezzo F, et al. 2005. J Pharmacol Exp Ther. 314:738-44. Sturn DH, et al. 2005. Microcirculation.12:393-403. Murdoch C, et al. 2007. J Immunol. 178:7405-11. Yuan HT, et al. 2009. Mol Cell Biol. 29:2011-22. Procopio WN, et al. 1999. J Biol Chem. 274:30196-201. Bhandari V, et al. 2006. Nature Med. 12:1286-93.
Gene ID: 285
UniProt: View information about Angiopoietin-2 on UniProt.org
Clone: A15124B
Regulatory Status: RUO
Other Names: Angiopoietin 2, ANG2, Ang-2, ANGPT2
Isotype: Mouse IgG2b, κ