Biolegend, 629852, Purified anti-Caldesmon Antibody, 100μg

Caldesmon is a calmodulin-, actin-, and tropomyosin-binding protein that plays an essential role in smooth muscle and non-muscle contraction. This protein is also involved in cellular mitosis and Schwann cell migration during peripheral nerve regeneration.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human Caldesmon
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunohistochemistry (IHC-P), either Citrate Buffer, 10X (Cat. No, 420902) or Tris-EDTA pH 9.0 is recommended for antigen retrieval. For immunocytochemistry (ICC), fixing with Fixation Buffer (Cat. No. 420801) is recommended. Alternatively, 4% paraformaldehyde fixation followed by either permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended. Clone W22071A cross-reacts with mouse in western blotting (WB).
RRID: AB_3083418 (BioLegend Cat. No. 629851) AB_3083418 (BioLegend Cat. No. 629852)
Structure: Caldesmon is a 793 amino acid protein with a predicted molecular mass of 93 kD
Distribution: Tissue distribution: high-molecular-weight Caldesmon (isoform 1) is predominantly expressed in smooth muscle; low-molecular-weight Caldesmon (isoforms 2, 3, 4 and 5) is widely distributed in non-muscle tissues and cells. Caldesmon is not expressed in skeletal muscle or heart. Cellular distribution: cytoskeleton, cytosol, and plasma membrane.
Function: Regulates actomyosin interactions in smooth muscle and non-muscle cells which affects cell contractions; involved in cellular mitosis and in Schwann cell migration during peripheral nerve regeneration.
Interaction: Calmodulin, actin, and tropomyosin
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure
Molecular Family: Enzymes and Regulators
Antigen References: Huber PA. 1997. Int J Biochem Cell Biol. 29:1047-51. Mayanagi T and Sobue K. 2011. Cell. Adh. Migr. 5:150-9. Wang CL. 2008. Adv Exp Med Biol. 644:250-72. Sobue K and Sellers JR. 1991. J Biol Chem. 266:12115-8.
Gene ID: 800
UniProt: View information about Caldesmon on UniProt.org
Clone: W22071A
Regulatory Status: RUO
Other Names: Caldesmon 1, Caldesmon, CDM, H-CAD, L-CAD, H-CD, Testis Secretory Sperm-Binding Protein Li 227n, NAG22, LCAD, HCAD, CAD
Isotype: Rat IgG2a, κ