Biolegend, 629952, Purified anti-MTHFD1 Antibody, 100μg

MTHFD1 is a trifunctional protein containing 10-formyltetrahydrofolate synthetase, 5,10-methenyltetrahydrofolate cyclohydrolase and 5,10-methylenetetrahydrofolate dehydrogenase activities and involved in the tetrahydrofolate interconversion pathway, which is part of one-carbon (1C) metabolism. MTHFD1 consists of two major domains, an N-terminal containing the dehydrogenase and cyclohydrolase activities and a larger synthetase domain in the C-terminus. According to reports, fibroblasts of patients with MTHFD deficiency exhibited enriched MTHFD1 in the nucleus, leading to low rate of de novo dTMP synthesis. Mutations in the MTHFD1 gene in pregnant women are associated with increased risk of giving birth to children with neural tube defects, along with possible decreased fetal survival. As folate metabolism is involved in migraine pathophysiology, particularly migraine with aura, MTHFD1 is also involved in migraine pathogenesis.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial Recombinant human MTHFD1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verifiied
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The clone cross-reacts with mouse in western blot (WB). For immunocytochemistry (ICC), ice-cold methanol fixation is recommended. This product is not recommended for use in immunohistochemistry (IHC).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for Western Blotting and Immunocytochemistry.
RRID: AB_3097335 (BioLegend Cat. No. 629951) AB_3097335 (BioLegend Cat. No. 629952)
Structure: MTHFD1 protein is a 935 amino acid protein with a predicted molecular weight of 102 kD
Distribution: Ubiquitous
Function: Nucleotide synthesis
Cell Type: Fibroblasts
Biology Area: Cell Biology
Molecular Family: Enzymes and Regulators
Antigen References: Krajinovic M. 2008. PHARMACOGENOMICS. 9:829-32. Field MS. et al. 2014. Proc Natl Acad Sci USA. 112(2):400-5. Watkins D, et al. 2011. Journal of Medical Genetics. 48:590-2. Parle-McDermott A, et al. 2005. Mol Hum Reprod. 11:477-80. Sutherland HG, et al. 2014. Headache. 54:1506-14.
Gene ID: 4522
UniProt: View information about MTHFD1 on UniProt.org
Clone: W22086A
Regulatory Status: RUO
Other Names: C-1-tetrahydrofolate synthase, cytoplasmic; CIMAH; MTHFC; MTHFD
Isotype: Rat IgG2b, κ