Biolegend, 630102, Purified anti-PTBP1 Antibody, 100μg

The 3H7 monoclonal antibody recognizes human PTBP1 also known as Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, and 57 kD RNA-binding protein. PTBP1 is a ubiquitously expressed nuclear protein with a predicted molecular weight approximately 59.6 kD. The polypyrimidine tract binding protein (PTB) is an important regulator of alternative splicing that also affects mRNA localization, stabilization, polyadenylation, and translation. PTBP1 has been shown to interact with FUS, Caspase3, PKCa, Raver1, and HnRNP-L protein. The PTB gene contains 15 exons and spans 13.5 kb. The 3H7 antibody has been shown to be useful for Western blotting.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human PTBP1 (full length)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIHC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5ml antibody dilution buffer for each mini-gel. For IHC, use a 15 µg/ml dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Gabai VL, et al. 2012. Moll Cell Biol. 32:929. PubMed.
Product Citations: Gabai V, et al. 2012. Mol Cell Biol. 32:929. PubMed Pablos I, et al. 2021. Cell Rep. 37:109892. PubMed
RRID: AB_2171285 (BioLegend Cat. No. 630101)
Structure: PTBP1 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). PTBP1 contains four RNA recognition motifs (RRMs). Nuclear protein, predicted molecular weight approximately 59.6 kD
Distribution: Nucleus, ubiquitously expressed
Function: Plays a role in pre-mRNA splicing. Binds to the polypyrimidine tract of introns. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA.
Interaction: FUS, Caspase3, PKCa, Raver1, and HnRNP-L
Biology Area: Cell Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. GilA, et al. 1991. Genes Dev. 5 (7):1224.2. Ghetti A, et al. 1992. Acids Res. 20:3671.3. Romanelli MG, et al. 2000. Gene 255:267.
Gene ID: 5725
UniProt: View information about PTBP1 on UniProt.org
Clone: 3H7
Regulatory Status: RUO
Other Names: Polypyrimidine tract binding protein 1, Heterogeneous nuclear ribonucleoprotein I, hnRNP I, 57 kD RNA-binding protein PPTB-1, PTB
Isotype: Mouse IgG1, κ