Biolegend, 632552, Purified anti-CREB1 Phospho (Ser133) Antibody, 100μg

Cyclic AMP (cAMP)-response element-binding protein 1 (CREB1) is a phosphorylation-dependent transcription factor that binds to the cAMP response element (CRE) in response to certain stimuli. CREB1 contains a basic leucine zipper (bZIP) DNA-binding domain, 1 dimerization domain, and 9 serine residues in 1 kinase inducible domain phosphorylated by multiple kinases, including Ca2+/CaM-dependent kinase (CaMK) II and IV, protein kinase A (PKA), protein kinase C (PKC), mitogen/stress-activated kinase (MSK), ribosomal S6 kinase (RSK), AKT, and MAPKAP kinase 2 (MK2). Both the protein levels and activation of CREB1 are vital for maintaining cellular homeostasis in physiological and pathological conditions. Phosphorylation of CREB1 at Ser133 promotes the recruitment of co-activator proteins CBP and p300, which has been proposed to increase transcription of CREB-dependent genes. Ser133 can also be phosphorylated by other proteins such as MSK1/2 in response to the activation of MAPK signaling.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide for human CREB1 phosphorylated at serine 133
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, ICFC, IHC-P - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 10 μg/mL is recommended. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunocytochemistry (ICC), we recommend to fix and perm the cells with either PFA-triton X-100, or PFA-Methanol. Fixation/permeabilization with methanol-only is not a suitable method for this antibody.
RRID: AB_3097586 (BioLegend Cat. No. 632551) AB_3097586 (BioLegend Cat. No. 632552)
Structure: CREB1 contains 327 amino acids at a predicted molecular weight around 37 kD.
Distribution: Ubiquitously expressed/Nucleus
Function: Under physiological conditions, CREB1 maintains cellular survival, proliferation, anti-inflammatory immune responses and modulates the development of the nervous system by serving a neuroprotective role. Under pathological conditions, increased levels of CREB1 is associated with acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Hodgkin’s lymphoma, chronic lymphatic leukemia (CLL), melanoma, hepatocellular, renal cell, ovarian, prostate, lung, gastric, esophageal, pancreatic, and breast tumors. CREB1 is also involved in the aging process by regulating the downstream signals of mitochondria dysfunction.
Interaction: CREB1 interacts with CREBBP, CRTC2, EP300, CRTC1, JUN, AKT1, CAMK4, FOS, RPS6KA1, RPS6KA3, CaMKII, CaMKIV, PKA, PKC, MSK, RSK, AKT, and MK2.
Cell Type: B cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, NK cells, T cells
Biology Area: Cell Biology, Transcription Factors
Antigen References: Steven A, et al. 2020. Cell Mol Life Sci. 77:4049-4067. Lin PH, et al. 2020. Diagnostics (Basel). 10:295. Sakamoto K, et al. 2011. J Neurochem. 116:1-9. Wen AY, et al. 2010. J Immunol. 185:6413-9. Arnould T, et al. 2002. EMBO J. 21:53-63. Naqvi S, et al. 2014. Biochem J. 458:469-79.
Gene ID: 1385
UniProt: View information about CREB1 on UniProt.org
Clone: A22026B
Regulatory Status: RUO
Other Names: CAMP responsive element binding protein 1; CREB-1; Cyclic Adenosine 3',5'-Monophosphate Response Element-Binding Protein; CAMP-Responsive Element-Binding Protein 1
Isotype: Mouse IgG2b, κ