Biolegend, 633451, Purified anti-NeuroD1 Antibody, 25μg

Neurogenic differentiation factor 1, also known as NeuroD1, is essential in both the pancreas and nervous system development and plays an important role in the formation of the inner ear and mammalian retina.  NeuroD1 is a transcription factor that belongs to the basic helix-loop-helix (bHLH) protein family. These proteins interact with E-proteins by building heterodimers and binding to the conventional E-box sequence CANNTG. It regulates expression of the insulin gene, and the mutations of this gene affect type II diabetes mellitus in mouse models and in mortal clinical cases. Studies indicate that NeuroD1 also could be phosphorylated at S162, S259, S266, and S274 upon glucose stimulation in a Ca 2+ -dependent manner. In pancreatic β-cells, Erk phosphorylation increases NeuroD1 activity, but GSK-3β phosphorylation suppresses NeuroD1 in neurons. Within the adult cortex, NeuroD1 is a marker of mature excitatory pyramidal neurons within the upper-most layers of the cortex. Aberrations in NeuroD1 in the brain causes severe developmental abnormalities. Notably, the targeted mutation of NeuroD1 results in a profound loss of neurons in the cerebellum, hippocampal dentate gyrus, and the inner ear due to extensive cell death.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human NeuroD1 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For immunocytochemistry (ICC), 4% PFA fixation followed by permeabilization with 0.5% Triton X-100 or ice-cold methanol is recommended. This antibody (clone W22035C) does not cross-react with mouse and rat in western blotting (WB). This antibody (clone W22035C) is not suitable for use in immunohistochemistry (IHC).
Additional Product Notes: This antibody has been tested in knockout/knockdown models for WB and ICC.
RRID: AB_3662316 (BioLegend Cat. No. 633451) AB_3662316 (BioLegend Cat. No. 633452)
Structure: NeuroD1 protein is a 356 amino acid protein with a predicted molecular weight of 40 kD.
Distribution: Cytoplasm, Nucleus
Function: Transcriptional activator, differentiation factor required for dendrite morphogenesis and maintenance in the cerebellar cortex, binds to the insulin gene E-box
Interaction: TCF3/E47, EP300, RREB1, ATOH8
Cell Type: Neurons
Biology Area: Cell Biology, Cell Proliferation and Viability, Neuroscience, Transcription Factors
Antigen References: Chae J.H, et al. 2004. Mol Cells. 18:271-88. Schonhoff SE, et al. 2004. Endocrinology. 145:2639-44. Sharma A, et al. 1999. Mol Cell Biol. 19:704-13. Lee TY, et al. 2020. Exp Neurobiol. 29:189-206. Miyata T, et al. 1999. Genes Dev. 13:1647-52. Poulin G, et al. 1997. Mol Cell Biol.17:6673-82. Bormuth I, et al. 2013. J Neurosci.33:641-51. Malecki M. T, et al. 1999. Nature Genetics. 23:323-8. Tutukova S, et al. 2021. Front Mol Neurosci.14:662774.
Gene ID: 4760
UniProt: View information about NeuroD1 on UniProt.org
Clone: W22035C
Regulatory Status: RUO
Other Names: Neurogenic Differentiation Factor 1, BETA2, BHF-1, MODY6, Neurogenic Helix-Loop-Helix Protein NEUROD, Class A Basic Helix-Loop-Helix Protein, Beta-Cell E-Box Transactivator 2, Basic Helix-Loop-Helix Transcription Factor, BHLHA3, T2D
Isotype: Rat IgG2a, κ