Biolegend, 633508, PE anti-Bcl-2 Antibody, 100μg

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-x L , and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.
100μg
Verified Reactivity: Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: 0.2 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.
Application References(PubMed link indicates BioLegend citation): Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMedDoi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMedYamanaka, et al. 2003. J. Immunol. 170:816. (IHC)Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
Product Citations: Cheng MI, et al. 2023. Nat Immunol. 24:780. PubMed Ozga AJ, et al. 2022. Immunity. 55:82. PubMed Stravokefalou V, et al. 2023. Front Immunol. 13:1014802. PubMed Oda SK, et al. 2020. J Exp Med. 217: . PubMed Sung BY, et al. 2022. J Clin Invest. 132:. PubMed Singh K, et al. 2015. Sci Rep. 14:7767. PubMed Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed Van Braeckel-Budimir N, et al. 2018. Cell Rep. 24:3374. PubMed Goh W, et al. 2020. Cell Rep. 33:108285. PubMed Engel I, et al. 2016. Nat Immunol. 10.1038/ni.3437. PubMed Lehrke MJ, et al. 2021. Elife. 10:. PubMed Nakazawa Y, et al. 2021. Elife. 10:. PubMed Nabekura T, et al. 2020. Immunity. 96:52. PubMed Puleston D, et al. 2014. Elife. 3:3706. PubMed
RRID: AB_2043939 (BioLegend Cat. No. 633507) AB_2290367 (BioLegend Cat. No. 633508)
Structure: Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution: Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum
Function: Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification: Phosphorylation, Ubiquitination
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214. 2. Yang E, et al. 1995. Cell 80:285. 3. Huang Z, et al. 2000. Oncogene 19:6627. 4. Deng X, et al. 2003. Blood. 102:3179.
Regulation: Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID: 596
UniProt: View information about Bcl-2 on UniProt.org
Clone: BCL/10C4
Regulatory Status: RUO
Other Names: B-cell leukemia 2
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.