Biolegend, 634351, Purified anti-TFAP2A Antibody, 25μg

TFAP2A, or transcription factor AP-2-alpha, binds to a GC-rich DNA consensus sequence 5'-GCCNNNGGC-3'. Its functions are integral to numerous biological processes including embryonic ectodermal development, stem cell differentiation, and the regulation of cell growth and proliferation. TFAP2A is active in the neural crest and regulates a group of cells in the early embryo that give rise to many tissues and organs, including bones and other tissues of the head and neck. In humans, TFAP2A mutation causes Branchio-oculo-facial syndrome (BOFS). In kidney development, TFAP2A functions in distal nephron development dictating the progression of cells towards a fully differentiated state. TFAP2A is also involved in carcinogenesis and is highly expressed in various types of cancer, including bladder cancer, melanoma, head and neck squamous carcinoma, gastric cancer, ovarian cancer, lung adenocarcinoma, and nasopharyngeal carcinoma. In addition to its role in cancer, TFAP2A is a well-known regulator of craniofacial development and evolution.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of human TFAP2A
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.0625 - 0.5 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10 μg/mL is recommended. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.25 - 1.0 µg/mL is suggested. For intracellular flow cytometric staining, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody (clone W20235D) in immunohistochemistry on formalin-fixed paraffin embedded tissue (IHC-P), antigen retrieval with Tris-EDTA pH 9.0 or Citrate Buffer, 10X (Cat. No. 420902) is recommended. For use of this antibody (clone W20235D) in immunocytochemistry (ICC), fixation with either Fixation Buffer (Cat. No. 420801) followed by permeabilization with 0.5% Triton-X or 100% ice-cold methanol is recommended. For use of this antibody (clone W20235D) in intracellular flow cytometry (ICFC), fixation with Fixation Buffer (Cat. No. 420801) followed by True-Phos™ Perm Buffer (Cat. No. 425401) is recommended. This antibody (clone W20235D) is not recommended for mouse or rat reactivity.
RRID: AB_3106151 (BioLegend Cat. No. 634351) AB_3106151 (BioLegend Cat. No. 634352)
Structure: Forms homodimer or heterodimer with TFAP2B or TFAP2C to bind to DNA. Contains a PPxY motif which aids interaction with WWOX.
Distribution: Highly expressed in placenta, breast and skin. Located in cell nucleus.
Function: A transcription activator binds to the GC-rich sequence to regulate gene expression.
Interaction: WWOX, EP300, CITED2, and CITED4
Biology Area: Cell Biology, Cell Proliferation and Viability, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: Milunsky JM, et al. 2008. Am J Hum Genet. 82:1171-7. Wang WD, et al. 2011. Dev Biol. 360:173-85. Shi D, et al. 2014. Cancer Prev Res. 7:266-77.
Gene ID: 7020
UniProt: View information about TFAP2A on UniProt.org
Clone: W20235D
Regulatory Status: RUO
Other Names: Transcription factor AP-2-alpha, AP2-alpha, Activator protein 2, AP-2 transcription factor , AP-2, AP-2 alpha, AP2TF, TFAP2, TFAP2alpha, TFAP2 alpha, TFAP
Isotype: Rat IgG2a, κ