Biolegend, 635501, Purified anti-TCP-1β Antibody, 25μg

TCP-1β (Chaperonin containing T complex polypeptide 1, subunit 2) belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD. Chaperonin-containing TCP-1 is a large multisubunit complex of 800-900 kD, a protein complex considered to be the cytosolic homologue of mitochondrial HSP60. TCP-1β has been shown to interact with β-actin, 14-3-3, SUMO4, TCP-1α, β-Tubulin, α-Tubulin and CCT8. In vitro, TCP-1βmediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.Clone F39P7F11has been shown to be useful for western blotting and immunohistochemistry of human, mouse, rat and Frog TCP-1β.
25μg
Verified Reactivity: Human, Mouse, Rat, Frog
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Ovalbumin-conjugated synthetic Peptide RKRVPDHHPC
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: Upon receipt, store undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-10 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Coghlin C, et al. 2006. J Pathol. 210:351. PubMed
Product Citations: Coghlin C, et al. 2006. J Pathol. 210:351. PubMed Arines FM, et al. 2021. J Cell Biol. 220:. PubMed
RRID: AB_2275598 (BioLegend Cat. No. 635502)
Structure: Belongs to the TCP-1 chaperonin family, predicted molecular weight 57 kD.
Distribution: Cytoplasm
Function: In vitro, TCP-1β mediates the ATP-dependent folding of tubulin, actin, and α-transducin. This protein is also essential for cyclin E maturation and accumulation.
Interaction: β-actin, 14-3-3γ, SUMO4, TCP-1α, β-Tubulin, α-tubulin, CCT8
Biology Area: Cell Biology, Cell Motility/Cytoskeleton/Structure
Antigen References: 1. Yaffe MB, et al. 1992. Nature 358:245
Gene ID: 21454
UniProt: View information about TCP-1 beta on UniProt.org
Clone: F39P7F11
Regulatory Status: RUO
Other Names: CCT beta, CCTB, T-complex protein 1 subunit beta, TCP1 beta
Isotype: Mouse IgG2a, κ