Biolegend, 635852, Purified anti-RhoA Antibody, 100μg

RhoA, also known as Transforming protein RhoA, is a small GTPase that plays a crucial role in various cellular functions, such as cytoskeletal dynamics, cell migration, cell cycle regulation, and cellular development. It alternates between active and inactive states by binding to either GTP or GDP, respectively. It is primarily associated with the regulation of the cytoskeleton, particularly in the formation of actin stress fibers and actomyosin contractility. RhoA influences cell shape, polarity, and locomotion through its effects on actin organization. In human mesenchymal stem cells, RhoA activity switches the differentiation between adipogenesis or osteogenesis under actin-myosin-generated cytoskeletal tension via effector, Rho-kinases (ROCK). RhoA is essential for cleavage furrow formation and apical junctions in keratinocyte cell-cell adhesion. In the developing nervous system, p75NTR induces RhoA strengthening on actin assembly which limits growth cone mobility and neuronal elongation. RhoA is found overexpressed or hyperactivate in many types of cancers, including prostate cancer, gastric cancer, testis cancer, breast cancer, and chronic myeloid leukemia. A RhoA inhibitor targeting RhoA/Rho GTPase pathway aims to prevent breast cancer cell growth as a potential cancer drug.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant fragment of human RhoA
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody (clone A18230D) in immunocytochemistry (ICC), it is recommended to fix cells with Fixation Buffer (Cat. No. 420801) followed by permeabilization with ice-cold methanol or fixation and permeabilization with ice-cold methanol only. This antibody is not recommended for immunohistochemistry on formalin-fixed paraffin-embedded tissues (IHC-P).
RRID: AB_3106120 (BioLegend Cat. No. 635851) AB_3106120 (BioLegend Cat. No. 635852)
Structure: Contains two switch regions, Switch I and Switch II, modified by its activation status
Distribution: Expressed widely in most tissues/Located primarily in cytoplasm, may detect at low expression levels in nucleus
Function: GTPase which regulates cytoskeleton organization/Regulated by guanine nucleotide exchange factors (GEFs)
Interaction: ROCK1, ROCK2, ARHGEF2, ARHGEF3, ARHGDIA, ARHGDIB, ARHGEF28
Cell Type: Neurons, Platelets
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cardiovascular Biology, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Cell Structure, DNA Repair/Replication, Neuroscience, Synaptic Biology
Molecular Family: GTPases, Microtubules
Antigen References: Yamashita T, et al. 1999. Neuron. 24:585-93. McBeath R, et al. 2004. Dev Cell. 6:483-95. Pertz O, et al. 2006. Nature. 440:1069-72. Dubash AD, et al. 2011. PLoS One. 6:e17380. Shimokawa H, et al. 2016. Circ Res. 118:352-66. Tsubaki M, et al. 2021. Biomedicines. 9:35.
Gene ID: 387
UniProt: View information about RhoA on UniProt.org
Clone: A18230D
Regulatory Status: RUO
Other Names: Ras Homolog Family Member A, Transforming protein RhoA, Ras Homolog Gene Family, Member A, Aplysia Ras-Related Homolog 12, RHOH12, ARH12, ARHA, RHO12, Epididymis Secretory Sperm Binding Protein
Isotype: Mouse IgG1, κ