Biolegend, 635951, Purified anti-ALPL Antibody, 25μg

ALPL is the tissue-nonspecific member of the alkaline phosphatase protein family, which includes four members: the tissue-nonspecific (liver/bone/kidney) ALP also called TNSALP, encoded by ALPL gene, and three tissue-specific ALPs that include intestinal (IAP), placental (PLAP-1), and placental-like 2 or germ cell (GCAP) ALPs; these enzymes are encoded by ALPI, ALPP, and ALPPL2 genes, respectively. Both ALPL and ALPI are expressed in many vertebrate species, while PLAP-1 and GCAP are expressed only in mammals. ALPL catalyzes the dephosphorylation of compounds in many transphosphorylation reactions. ALPL hydrolyzes monophosphated esters such as phosphoethanolamine, inorganic pyrophosphate, and pyridoxal 5′-phosphate.  ALPL is a homodimeric protein and each monomer possesses 524 amino acids. It is expressed mainly in liver, bone, and kidney tissues, although it can be found in all tissues. It is typically found in a membrane bound form. Its catalytic site harbors two Zn ions and one Mg ion essential for enzyme activity. Mutations in the ALPL gene causing deficiency in ALPL activity result in hypophosphatasia and skeletal defects.  ALPL regulates senescence and differentiation in mesenchymal stem cells from bone marrow. ALPL deficiency induces bone mass loss and increase in marrow fat, characteristics of premature bone ageing. ALPL was found to regulate cancer cell motility in some ovarian cancers via a non-canonical WNT pathway.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human ALPL protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IP - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/mL is recommended. For immunoprecipitation, the suggested use of this reagent is 5.0 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This antibody (clone W20113I) does not cross-react with mouse ALPL. This product is not suggested for use in mouse. For immunocytochemistry (ICC), we recommend using 4% PFA fixation followed by permeabilization with 0.5% Triton X-100. This antibody (clone W20113I) is not suitable for immunohistochemistry (IHC).
Additional Product Notes: This antibody (clone W20113I) has been tested in knockout/knockdown models for WB and ICC.
RRID: AB_3106113 (BioLegend Cat. No. 635951) AB_3106113 (BioLegend Cat. No. 635952)
Structure: ALPL is a 524 amino acid protein with a predicted molecular weight of 57 kD.
Distribution: Osteoblast, bone marrow mesenchymal stem cells, liver, bone, kidney
Function: Bone and tooth mineralization, metabolism of nucleotides, sequentially hydrolyzes ATP, ADP and AMP
Interaction: Monoesters of phosphoric acid, inorganic pyrophosphate, phosphoethanolamine, and pyridoxal 5′-phosphate. Interacts with the ECM, collagen
Cell Type: Mesenchymal Stem Cells, Osteoblasts
Biology Area: Cell Biology, Stem Cells
Molecular Family: Protein Kinases/Phosphatase
Antigen References: Weis MJ, et al. 1988. J Biol Chem. 263:12002-10. Kim EE and Wyckoff HW. 1991. J Mol Biol. 218:449-64. Mornet E. 2000. Human Mutation. 15:309-15. Yang Y, et al. 2012. Front Immunol. 3:314. Silvent J, et al. 2014. J Biol Chem. 289:24168-79. Liu W, et al. 2018. Bone Res. 6:27. Lou M, et al. 2019. Biochem Biophys Res Commun. 513:528-533.
Gene ID: 249
UniProt: View information about ALPL on UniProt.org
Clone: W20113I
Regulatory Status: RUO
Other Names: AP-TNAP, TNAP, Tissue-Nonspecific ALP, APTNAP, Alkaline Phosphatase, Biomineralization Associated; Alkaline Phosphatase, Tissue-Nonspecific Enzyme (TNAP); Alkaline Phosphatase, Liver/Bone/Kidney Isozyme
Isotype: Rat IgG2b, κ