Biolegend, 637651, Purified anti-ZO-3 Antibody, 25μg

ZO-3 is a member of the membrane-associated guanylate kinase-like (MAGUK) protein family. Together with ZO-1 and ZO-2, they act as scaffold proteins that link transmembrane proteins in the tight junctions such as claudins, junctional adhesins and occludins to the actin cytoskeleton forming a boundary between the apical and basolateral plasma membrane on epithelial cells. The importance of this complex of proteins is to maintain tight junctions and limit the paracellular movement of substances. In addition, ZO-3 sequesters Cyclin D1 avoiding its degradation during mitosis and enabling S-phase transition. Celiac Disease 1 has been associated with ZO-3.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Partial recombinant TJP3 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3106184 (BioLegend Cat. No. 637651) AB_3106184 (BioLegend Cat. No. 637652)
Structure: ZO-3 is a 130 kDa protein with multiple PDZ motifs, one SH3 domain, and one guanylate kinase-like domain.
Distribution: Tight junctions in the cytoplasmic side of the plasma membrane, nucleus
Function: ZO-3 is part of the protein complex that maintains the boundary between the apical and basolateral plasma membrane domains of epithelial and endothelial cells. This complex of proteins also limits movement of substances through the paracellular space.
Interaction: ZO-1, ZO-2, Claudins, transmembrane adhesins, actin cytoskeleton, Cyclin D1
Cell Type: Endothelial cells, Epithelial cells
Biology Area: Cell Biology, Cell Structure
Molecular Family: Adaptor Proteins, Adhesion Molecules, Gap Junction Proteins, Scaffold Proteins
Antigen References: Haskins J, et al. 1998. J Cell Biol. 141:199-208 Adachi M, et al. 2006. Mol Cell Biol. 26:9003-9015. Itoh M, et al. 1999. J Cell Biol. 147:1351-1363. Zhuang X, et al. 2023. Biomedicines. 11:3136.
Gene ID: 27134
UniProt: View information about ZO-3 on UniProt.org
Clone: W22143C
Regulatory Status: RUO
Other Names: Tight Junction Protein ZO-3, Zonula Occludens protein 3, Zona Occludens Protein 3, ZO3, Zona Occludens 3
Isotype: Rat IgG2a, κ