Biolegend, 639955, Alexa Fluor® 647 anti-CD340 (ErbB2/HER-2) Antibody, 25μg

CD340, also commonly known as HER-2 or ErbB2, is a member of the epidermal growth factor receptor family of cell membrane tyrosine kinases containing a single transmembrane domain. CD340 contains three furin repeats and two CheY homologous receiver domains in the extracellular region. Although CD340 itself contains no ligand- binding domain, it interacts with other EGF receptor family members to form a heterodimer, stabilize ligand binding, and enhance kinase-mediated downstream signaling. CD340 has been shown to be involved in embryonic development and cancer progression; this protein is amplified in lung adenocarcinoma, some breast cancers, glioblastoma, gastric cancer, and ovarian carcinoma, where it functions as an oncogene. CD340 is a therapeutic target in several human cancers and it has been shown that overexpression in breast cancers confers Taxol resistance.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant fragment of the intracellular domain of human CD340 (ErbB2/HER-2)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: IHC-P - Quality tested ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.0 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. * Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Additional Product Notes: For use in intracellular flow cytometry (ICFC), it is recommended to fix/permeabilize with Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803). Fixation/permeabilization with True-Phos™ Perm Buffer (Cat. No. 425401) or True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) is not recommended. For use in immunohistochemistry on formalin-fixed paraffin-embedded tissue (IHC-P), it is recommended to perform antigen retrieval using either Citrate Buffer, 10X (Cat. No. 420902) or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (10X) (Cat. No. 422704). For use in immunocytochemistry (ICC), it is recommended to fix/permeabilize with any of the following: -Fixation buffer (Cat. No. 420801) with 0.5 % Triton X-100 -Fixation buffer (Cat. No. 420801) with 100% ice-cold methanol Fixation/permeabilization with 100% ice-cold methanol only is not recommended.
RRID: AB_3720625 (BioLegend Cat. No. 639955) AB_3720625 (BioLegend Cat. No. 639956)
Structure: CD340 is a 1255 amino acid protein with a predicted molecular weight of 137.9 kD. It is a glycosylated receptor-like transmembrane protein; due to glycosylation, the observed molecular weight by Western Blot is closer to 175-185 kD.
Distribution: Cellular membrane and also present in internalized vesicles
Function: Receptor, transcriptional regulator
Interaction: Homodimerizes Heterodimerizes with EGFR, ErbB3, ErbB4, PIK3C2B, KPNB1, RANBP2, EEA1, CRM1, CLTC, MUC1, PTK6, RPA194, ACTB, PRKCABP, SRC, MYOC, CPNE3, SORL1, SH3BGRL
Ligand/Receptor: Heterodimerizes with other ERBB family members upon ligand binding, but does not directly bind ligand
Cell Type: Leukemia
Biology Area: Cell Biology, Immunology, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Akiyama T, et al. 1986. Science. 232:1644-6. Bargmann CI, et al. 1986. Nature. 319:226-30. Pegram MD, et al. 1997. Oncogene. 15:537-47. Slamon DJ, et al. 1989. Science. 244:707-12. Leone F, et al. 2003. J Leukoc Biol. 74:593-601.
Gene ID: 2064
UniProt: View information about CD340 on UniProt.org
Clone: W22266D
Regulatory Status: RUO
Other Names: Receptor tyrosine-protein kinase ErbB-2 (ERBB2), human epidermal growth factor receptor 2 (HER2), metastatic lymph node gene 19 protein (MLN19), Proto-oncogene Neu, p185(erbB2), cluster of differentiation 340 (CD340), NGL, herstatin
Isotype: Rat IgG2a, κ