Biolegend, 640451, Purified anti-Rb Phospho (Ser795) Antibody, 25μg

The Retinoblastoma protein (Rb) is a tumor suppressor that regulates cell cycle progression from G1 phase into the S phase. This regulation is crucial for preventing uncontrolled cell proliferation, which can lead to cancer. Hence, mutations or dysfunctions in the RB1 gene are associated with several cancers including retinoblastoma, lung, bladder, and breast cancers. Phosphorylation at serine 795 of the Rb protein plays a crucial role in regulating its function. The serine 795 phosphorylation leads to Rb dissociation from E2F transcription factor and allowing E2F to induce the transcription of cell cycle genes. This regulatory mechanism is vital for normal cell function and its dysregulation can contribute to cancer development.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide of human Rb phosphorylated at serine 795
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Additional Product Notes: For use of this antibody in intracellular flow cytometry (ICFC), we recommend fixation and permeabilization using True-Phos™ Perm Buffer (Cat. No. 425401). Similar results were also obtained using True-Nuclear™ Transcription Factor Buffer set (Cat. No. 424401) or Cyto-Fast™ Fix-Perm Buffer set (Cat. No. 426803). For use of this antibody in Immunocytochemistry (ICC), we recommend using PBS-Tween-20 0.05% as a wash buffer. For use of this antibody in immunohistochemistry on formalin-fixed paraffinembedded tissues (IHC-P), we recommend antigen retrieval using Citrate Buffer (Cat. No. 420902), or Tris-EDTA pH 9.0 Antigen Retrieval Buffer (Cat. No. 422704).
RRID: AB_3699068 (BioLegend Cat. No. 640451) AB_3699068 (BioLegend Cat. No. 640452)
Structure: The Rb protein is 928 amino acids in length and has a molecular weight of approximately 106 kDa.
Distribution: Nucleus, cytoplasmic during keratinocyte differentiation
Function: • Tumor Suppression: Rb acts as a tumor suppressor by inhibiting excessive cell growth and proliferation. • Cell Cycle Regulation: Rb controls progression from the G1 phase to the S phase of the cell cycle by binding to and inhibiting E2F transcription factors. • Transcription Regulation: Rb regulates the transcription of genes necessary for DNA synthesis and cell cycle progression. • Apoptosis: Rb can also induce apoptosis under certain conditions, contributing to its role in preventing cancer.
Interaction: • E2F Transcription Factors: Rb binds to E2F transcription factors, inhibiting their activity and preventing the transcription of genes required for cell cycle progression. • DP Proteins: Rb also interacts with DP proteins, which form heterodimers with E2F, enhancing the regulation of E2F activity. • Cyclin-Dependent Kinases (CDKs): CDKs phosphorylate Rb, leading to its inactivation and allowing the cell cycle to progress. • Histone Deacetylases (HDACs): Rb recruits HDACs to E2F target genes, leading to chromatin remodeling and transcriptional repression. • Chromatin Remodeling Enzymes: Rb interacts with various chromatin remodeling enzymes, such as methylases and acetylases, to regulate gene expression.
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, DNA Repair/Replication, Signal Transduction, Transcription Factors
Molecular Family: Phospho-Proteins, Tumor Suppressors
Antigen References: Rezaeian A, et al. 2023. Adv Protein Chem Struct Biol. 135:179. Janostiak R, et al. 2022. Cancers (Basel). 14:1265. Indovina P, et al. 2015. Oncotarget. 6:17873. Linn P, et al. 2021. Cancers (Basel). 13:3737.
Regulation: • Phosphorylation: Rb is regulated by phosphorylation, particularly by cyclin-dependent kinases (CDKs). Phosphorylation at specific sites, such as serine 795, inactivates Rb, allowing cell cycle progression. • Dephosphorylation: Dephosphorylation of Rb reactivates its tumor suppressor functions, inhibiting cell cycle progression. • Gene Mutations: Mutations in the RB1 gene can lead to loss of Rb function, contributing to the development of cancers such as retinoblastoma, lung, bladder, and breast cancers.
Gene ID: 5925
UniProt: View information about Rb Phospho Ser795 on UniProt.org
Clone: A24001E
Regulatory Status: RUO
Other Names: RB1, Retinoblastoma-associated protein, p105-Rb, p110-RB1
Isotype: Mouse IgG1, κ