Biolegend, 641401, Purified anti-c-Fos Antibody, 25microl

The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1.
25microl
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Polyclonal
Host Species: Rabbit
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
Preparation: The antibody was purified by antigen-affinity chromatography.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: Upon receipt, store at -20°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 - 1:4000 antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product may contain other non-IgG subtypes. This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
Application References(PubMed link indicates BioLegend citation): Fan W, et al. 2009. Brain Res Red. 59:324. PubMed Sugimoto K, et al. 2014. Neurosci Res. PubMed
Product Citations: Cong J, et al. 2022. Theranostics. 12:5204. PubMed Chung H, et al. 2021. Nat Methods. 18:1204. PubMed Sugimoto K, et al. 2015. Neurosci Res. 96: 20-29. PubMed Fan W, et al. 2009. Brain Res Rev. 59:324. PubMed
RRID: AB_2106632 (BioLegend Cat. No. 641401) AB_2106629 (BioLegend Cat. No. 641402)
Structure: c-fos heterodimerizes with c-jun to form the transcription factor AP-1; contains a bZIP region with a DNA-binding domain and a leucine zipper domain; approximately 62 kD.
Distribution: Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes.
Function: FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal form
Interaction: Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Modification: Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Synaptic Biology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Dony C, et al. 1987. Nature 328:711. 2. Grigoriadis AE, et al. 1994. Science 266:443. 3. Glover JNM, et al. 1995. Nature 373:257.
Regulation: c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia.
Gene ID: 2353
UniProt: View information about c-Fos on UniProt.org
Clone: Poly6414
Regulatory Status: RUO
Other Names: Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog
Isotype: Rabbit Polyclonal IgG