Biolegend, 643653, Ultra-LEAF™ Purified anti-human BMP-6 Antibody, 100μg

Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. BMPs play a key role in embryonic development, especially during heart, neural, and cartilage development. Around 20 BMP family members have been identified and characterized. BMPs signal through serine/threonine kinase receptors, composed of type I and II subtypes. Four type I receptors have been identified: type IA and IB BMP receptors, type IA activin receptor, and activin receptor-like kinase I (ALK-1). Three type II receptors have also been recognized: type II BMP receptor and type II and IIB activin receptors. Human recombinant BMP-6 can restore bone in animal models of osteoporosis. BMP-6 induces bone formation through IGF-I and EGF pathways in a murine model of osteopenia and in human osteoblasts. BMP-6 has been reported to have an antiproliferative effect on B, T cells, and macrophages. Also, BMP-6 induced expression of pro-inflammatory inducible nitric oxide synthase (iNOS), TNF-α and IL-1β in macrophages. In addition, BMP-6 is a key endogenous regulator of hepcidin expression and iron metabolism in vivo. In fact, BMP-6 null mice have a phenotype that resembles mouse models of juvenile hemochromatosis (an iron overload disorder caused by mutations in the genes encoding the major iron regulatory hormone hepcidin and hemojuvelin).
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant Human BMP-6
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: Neut - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by neutralizing the differentiation induced by recombinant human BMP-6 (Cat. No. 595502) at 0.1 µg/mL on ATDC5 mouse chondrogenic cells. ND50 range: 0.1 - 0.6 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3662325 (BioLegend Cat. No. 643653) AB_3662325 (BioLegend Cat. No. 643654)
Structure: Growth factor, Homodimer
Distribution: Hepatocytes
Function: Osteoblast differentiation, bone regeneration. Antiproliferative effect on B, T cells, and macrophages. BMP-6 regulates hepcidin expression and iron metabolism in vivo.
Interaction: Cancer cells, mesenchymal stem cells germline stem cells, hematopoietic stem cells, and neural stem cells
Ligand/Receptor: BMP receptors Alk-2 and Alk-3 (type I), BMPRII (type II)
Cell Type: Embryonic Stem Cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area: Angiogenesis, Cell Biology, Neuroscience, Stem Cells, Synaptic Biology
Molecular Family: Cytokines/Chemokines, Growth Factors
Antigen References: Chen D, et al. 2004. Growth Factors. 22:233-41. Grasser WA, et al. 2007. Int Orthop. 31:759-65. Sivertsen EA, et al. 2007. Eur J Immunol. 37:2937-48. Andriopoulos B, et al. 2009. Nat Genetics. 41:482-7. Hong JH, et al 2009. Immunology. 128:e442-50. Lee GT, et al. 2011. Mol Immunol. 48:1540-7.
Gene ID: 654
UniProt: View information about BMP-6 on UniProt.org
Clone: A20139D
Regulatory Status: RUO
Other Names: Bone morphogenetic protein 6, VGR1
Isotype: Mouse IgG2a, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.