Biolegend, 644102, Purified anti-CD247 (TCRζ, CD3ζ) Antibody, 100μg

The invariant TCR zeta chain is a member of the CD3 complex associated with the clonotypic alpha/beta TCR heterodimer. The disulfide-linked TCR zeta homodimers transmit signals following TCR ligation. Loss of TCR zeta expression has been reported in a diverse range of disease states, including autoimmune diseases, many neoplastic conditions and chronic infections, such as tuberculosis and leprosy.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human TCR-zeta peptide
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICFC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.5-1 µg per ml antibody dilution buffer. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Zhang Z, et al. 2007. Blood 109:4328. (Block) Gorman CL, et al. 2008. J. Immunol. 180:1060. Jenson WA, et al. 1997. Eur J. Immunol. 27:707. Mao H, et al. 2010. J. Virol. 84:4148. PubMed Hwang I, et al. 2012. Int Immunol. 24:793. PubMed.
Product Citations: Wang X, et al. 2023. EMBO J. e110597:42. PubMed Ghosh S, et al. 2020. Cell Reports. 30(10):3434-3447. PubMed Hwang I, et al. 2012. Int Immunol. 24:793. PubMed Ye Z, et al. 2021. Nat Commun. 12:907. PubMed Mao H, et al. 2010. J Virol. 84:4148. PubMed
RRID: AB_1575006 (BioLegend Cat. No. 644101) AB_2072963 (BioLegend Cat. No. 644102)
Disclaimer: This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.
Structure: Structure: 163 amino acid long protein (predicted MW is 18kDa), with a short extracellular domain of only nine amino acids conserved between mouse and human. There are 6-7 intracellular tyrosines that are potential phosphorylation sites.
Distribution: T cells and NKT cells. Reported also positive on CD16+ human NK cells.
Function: Mediating signal transduction after TCR receptor engagement.
Interaction: ZAP70, TCR alpha/beta
Cell Type: NKT cells, T cells
Biology Area: Cell Biology, Immunology, Signal Transduction
Molecular Family: CD Molecules, TCRs
Antigen References: 1.Weissman A, et al. 1988. P. Natl. Acad. Sci. USA 85:9709
Gene ID: 919
UniProt: View information about CD247 on UniProt.org
Clone: 6B10.2
Regulatory Status: RUO
Other Names: CD247, CD3zeta
Isotype: Mouse IgG1, κ