Biolegend, 644702, Purified anti-GFAP Antibody, 100μg

GFAP is expressed exclusively in astrocytes in the central nervous system. The protein is a member of the intermediate filament family of proteins which form networks providing support and strength to cells. This antibody does not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin and others. More than 50 GFAP mutations have been identified to be associated with the Alexander disease.
100μg
Verified Reactivity: Human, Mouse, Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Bovine spinal cord homogenate
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested ICC, IHC-F, IHC-P - Verified FC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.25 - 1.0 µg antibody per mL antibody dilution buffer for each mini-gel. For immunocytochemistry, a concentration range of 1.0 - 5.0 µg/mL is recommended. For immunohistochemical staining of frozen tissue sections, a concentration range of 5.0 - 10 µg/mL is suggested. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): McLendon RE and Bigner DD. 1994. Brain Pathol. 4:221. Liu W, et al. 2011. Proteomics 11:3556. (FC) PubMed
Product Citations: Yamaguchi N, et al. 2023. IBRO Neurosci Rep. 14:253. PubMed Salgado B, et al. 2023. Microorganisms. 11:. PubMed Liu D, et al. 2022. Eur J Neurosci. 56:3553. PubMed Whitmore CA, et al. 2022. Pharmaceuticals (Basel). 15: . PubMed Mogas Barcons A, et al. 2022. Materials (Basel). 16: . PubMed Tao W, et al. 2020. EMBO Mol Med. 12:e12291. PubMed Eisemann T, et al. 2018. BMC Cancer. 18:103. PubMed Liu CL, et al. 2018. Mol Med Rep. 9:2359. PubMed Gerasimov E, et al. 2021. Int J Mol Sci. 22:. PubMed Shi Y, et al. 2016. Cell Death Differ. 10.1038/cdd.2016.110. PubMed Gómez-Gálvez Y, et al. 2020. J Neurosci Res. 1417:98. PubMed Ivanova EL, et al. 2022. Front Oncol. 12:969787. PubMed Costa B, et al. 2021. Cancers (Basel). 13:00. PubMed Moyon S, et al. 2021. Nature Communications. 12(1):3359. PubMed Marcelo A, et al. 2021. Cell Death Dis. 12:1117. PubMed Wang YJ, et al. 2021. Sci Rep. 11:8054. PubMed Chompre G, et al. 2022. Heliyon. 8:e11194. PubMed Murenu E, et al. 2021. Int J Mol Sci. 22:. PubMed Costa B, et al. 2019. Blood Adv. 3:1092. PubMed
RRID: AB_2109791 (BioLegend Cat. No. 644701) AB_2294566 (BioLegend Cat. No. 644702)
Structure: Around 50 kD
Distribution: Only expressed in astrocytes in the central nervous system.
Function: Together with other proteins to form intermediate filaments which supports astroglial cells. Astroglial cells support and nourish cells in the brain and spinal cord. If brain cells are injured through trauma or disease, astroglial cells react by rapidly prodcing more GFAP protein.
Cell Type: Astrocytes
Biology Area: Cell Biology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Intermediate Filaments
Gene ID: 2670
UniProt: View information about GFAP on UniProt.org
Clone: 2E1.E9
Regulatory Status: RUO
Other Names: Glial fibrillary acid protein
Isotype: Mouse IgG2b