Biolegend, 644801, Purified anti-T-bet Antibody, 25μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
25μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB, ICFC - Quality tested IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting and intracelluar immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For Western blotting, the suggested use is 1.0 to 2.0 µg per ml. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a staining volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Yang Z, et al. 2022. Cancer Discov. 12:1942. PubMed Poon MML, et al. 2023. Nat Immunol. 24:309. PubMed Stankovic S, et al. 2015. J Immunol. 195: 4892 - 4899. PubMed Seifert HA, et al. 2018. Metab Brain Dis. 33:1599. PubMed Wang D, et al. 2018. Immunity. 48:659. PubMed Arnold IC, et al. 2018. J Exp Med. 215:2055. PubMed Yang J, et al. 2020. Front Immunol. 10:3048. PubMed Draganov D, et al. 2021. NPJ Breast Cancer. 7:22. PubMed Chen J, et al. 2020. J Immunol. 204:2076. PubMed Wang Y, et al. 2021. Cancer Cell. 39:1375. PubMed Mayer KA, et al. 2021. FASEB J. 35:e21217. PubMed Hilligan KL, et al. 2020. Nat Commun. 11:5637. PubMed Hsieh C, et al. 2012. J Pharmacol Exp Ther. 343:125. PubMed Zhao L, et al. 2018. Nat Med. 24:1536. PubMed Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed Yang K, et al. 2017. Nature. 548:602. PubMed O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed Sprouse ML, et al. 2018. JCI Insight. 3:e97322. PubMed Tao H, et al. 2021. Front Immunol. 12:623280. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Ejima R, et al. 2021. Nutrients. 13:. PubMed Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed Kakaradov B, et al. 2017. Nat Immunol. 18:422. PubMed Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed Newell KL, et al. 2021. PLoS One. 16:e0244855. PubMed Roussey JA, et al. 2017. J Immunol. 199:3535. PubMed Wang D, et al. 2022. EMBO Rep. 23:e53691. PubMed Scortegagna M, et al. 2020. Nat Commun. 11:99. PubMed Stelekati E, et al. 2018. Cell Rep. 2.445833333. PubMed Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed Dey P, et al. 2020. Cancer Discov. 10:608. PubMed Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed Sasawatari S, et al. 2020. J Immunol. 1373:204. PubMed
RRID: AB_1595608 (BioLegend Cat. No. 644801) AB_1595503 (BioLegend Cat. No. 644802)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ