Biolegend, 644808, Pacific Blue™ anti-T-bet Antibody, 100μg

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.
100μg
Verified Reactivity: Human, Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 1.0 µg per million cells in a volume of 100 µl. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).
Application References(PubMed link indicates BioLegend citation): Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB) Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP) Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF) Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations: Campisi L, et al. 2022. Nature. 606:945. PubMed Liu F, et al. 2016. PLoS Pathog. 12: 1005663. PubMed Kalekar L, et al. 2016. Nat Immunol. 17:304-314. PubMed Dimova T, et al. 2015. Proc Natl Acad Sci U S A. 112:556. PubMed Bellavance E, et al. 2011. J Immunol. 186:3309. PubMed Schiller M, et al. 2021. Immunity. 54(5):1022-1036.e8. PubMed Ma C, et al. 2018. Science. 360:eaan5931. PubMed Chen Z et al. 2019. Immunity. 51(5):840-855 . PubMed Domingo–Gonzalez R, et al. 2020. eLife. 9:e56890.. PubMed Hanasoge Somasundara AV, et al. 2021. Cell Rep. 37:110099. PubMed Chen Z, et al. 2021. Cell. 184(5):1262-1280.e22. PubMed Rosário A, et al. 2012. J Immunol. 188:1178. PubMed Webb LM, et al. 2020. J Clin Invest. 130:1683. PubMed Jain A, et al. 2020. Nat Immunol. 0.920138889. PubMed
RRID: AB_1595586 (BioLegend Cat. No. 644807) AB_1595479 (BioLegend Cat. No. 644808)
Structure: T-box transcription factor, approximately 58 kD.
Distribution: Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.
Function: Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type: B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Transcription Factors
Molecular Family: Nuclear Markers
Antigen References: 1. Szabo SJ, et al. 2000. Cell 100:655. 2. Szabo SJ, et al. 2002. Science 295:338. 3. Finotto S, et al. 2002. Science 295:336. 4. Mullen AC, et al. 2001. Science 292:1907.
Gene ID: 30009
UniProt: View information about T-bet on UniProt.org
Clone: 4B10
Regulatory Status: RUO
Other Names: T-box expressed in T cells, T box 21, TBLYM
Isotype: Mouse IgG1, κ