Biolegend, 644901, Purified anti-RORγ Antibody, 25μg

RORγ (Retinoid-related orphan receptor gamma) belongs to the nuclear hormone receptor family, NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. RORγ has two isoforms: γ1 and γ2 (also referred to as RORγt). The RORγt differs from the RORγ1 isoform in that it lacks the amino terminus of RORγ1. RORγ1 contains 516 amino acids and RORγt contains 495 amino acids. RORγ1 has a molecular weight of approximately 58 kD. RORγ1 is highly expressed in thymus, kidney, liver, muscle, and brown fat but not in white fat tissue. RORγt is specifically expressed in only two cell populations, DP thymocytes and lymphoid tissue inducers (LTi). RORγ plays a critical role in control apoptosis during thymopoiesis and T cell homeostasis. RORγ is to regulate TCRα repertoire by virtue of its positive regulatory role on Bcl-x expression. RORγ is essential for lymph nodes and Peyers patches development.
25μg
Verified Reactivity: Human
Reported Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human RORγ-GST recombinant protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution: 1 to 4 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: This product weakly reacts with Mouse.
Application References(PubMed link indicates BioLegend citation): Klemann C, et al. 2009. Am J Pathol. 174:2234. PubMed
Product Citations: Hilt ZT, et al. 2019. Aging (Albany NY). 11955:11. PubMed Klemann C, et al. 2009. Am J Pathol. 174:2234. PubMed
RRID: AB_1595502 (BioLegend Cat. No. 644902)
Structure: Belongs to the nuclear hormone receptor family, NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. RORγ has two isoforms: γ1 and γ2 (also referred to as RORγt). The RORγt differs from the RORγ1 isoform in th
Distribution: Th17 cells
Function: RORγt is essential for lymph nodes and Peyer's patches development. RORγt plays a critical role in control apoptosis during thymopoiesis and T cell homeostasis.
Cell Type: Th17
Biology Area: Cell Biology, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Medvedev A, et al. 1997. Genomics 46:93. 2. He YW, et al. 1998. Immunity 9:797. 3. Eberl G, et al. 2004. Nat. Immunol. 5:64.
Gene ID: 19885
UniProt: View information about RORgamma on UniProt.org
Clone: 4F3-3C8-2B7
Regulatory Status: RUO
Other Names: RORc, Nuclear receptor ROR-gamma, Nuclear receptor RZR-gamma
Isotype: Mouse IgG1, κ