Biolegend, 645102, Purified anti-Caspase-1 Antibody, 100μg

Caspase -1 plays a vital role in cleaving the inactive procytokine pro-IL-1 beta to generate the active 17 kd mature form of IL-1beta, a key mediator of inflammation. Active caspase-1 (ICE) is derived a single proenzyme of 45 Kd. An active ICE comprises two subunits of P20 and two subunits of P10 after removal a large amino-terminal pro-domain and a small linker peptide. Caspase-1 is reported to be involved in CD95- mediated apoptosis. Four alternatively spliced variants of caspase-1 have been reported, with mw ranging from 30-48 kd.
100μg
Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Bacterial fusion protein (p20)
Formulation: This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1-2µg antibody per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Kang SJ, et al. 2000. J Cell Biol 149:613 Kang SJ, et al. 2002. Cell Death and Differentiation. 9:1115
Product Citations: Zhao YJ, et al. 2022. Neural Regen Res. 17:1324. PubMed Liu L, et al. 2017. J Crohns Colitis. 10.1093/ecco-jcc/jjw219. PubMed Zhu Q, et al. 2016. Am J Physiol Renal Physiol. 310: F621-F627. PubMed Reis AS, et al. 2020. Sci Adv. 6:eaax6346. PubMed Nastase MV, et al. 2016. The American Journal of Pathology. 186(5):1206-20. PubMed
RRID: AB_2068901 (BioLegend Cat. No. 645101) AB_2068900 (BioLegend Cat. No. 645102)
Structure: The whole length of Caspase-1 is a 404 amino acid polypeptide chain with MW of 45kd. The active Caspase -1 is a tetramer compariseing two subunits of 20Kd and 10Kd.
Distribution: Predominantly in the cytoplasm, some localized to the external cell membrane.
Function: Caspase-1 is a mediator of the inflammatory response by cleaving the inactive pro-IL1 beta at Asp116/Ala 117 to generate the active 17k mature form of IL-1 beta.
Ligand/Receptor: Pro-IL1 beta
Bioactivity: IL-1beta converting enzyme (ICE)
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References: 1. Wilson KP et al. 1994. Nature 370:251 2. Cohen GM:1997 Biochem J 326:1
Gene ID: 834
UniProt: View information about Caspase-1 on UniProt.org
Clone: 5B10
Regulatory Status: RUO
Other Names: ICE
Isotype: Rat IgG2a, κ