Biolegend, 645952, Purified anti-p21 Antibody, 100μg

p21 (also known as CIP1 or CDKN1A) is a G1 cell cycle regulator that inhibits cyclin-cyclin dependent kinase 2 and/or cyclin-cyclin dependent kinase 4 complexes. Its expression is highly controlled by the tumor suppressor protein p53, thus, p21 is involved in controlling the G1 cell cycle arrest in response to different stress stimuli. p21 plays a regulatory role in S phase DNA replication by interacting with PCNA (proliferating cell nuclear antigen). Activation of cyclin-dependent kinase 2 has been observed after p21 is cleaved by CASP3-like caspases. Loss of function of p21 has been associated with bladder cancer, Multiple Endocrine Neoplasia Type I and lymph node metastasis. In addition, p21 can bind the HIV integrase mediating resistance to infection in hematopoietic cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant protein fragment of p21
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality tested IHC-P, ICC, ICFC - Verified
Recommended Usage: Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 1.25 - 10.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: For use of this antibody (clone W22006E) in immunocytochemistry (ICC), we recommend fixation/permeabilization with either PFA/Triton X-100 or PFA/Methanol. This antibody is not suitable for use with methanol fixation/permeabilization.
RRID: AB_3662327 (BioLegend Cat. No. 645951) AB_3662327 (BioLegend Cat. No. 645952)
Structure: p21 is a 164 amino acid length protein with an approximate molecular weight of 18 kD.
Distribution: Nuclear localization; in some diseases, it is localized in the cytoplasm.
Function: Control of cell cycle progression Inhibition of cyclin-dependent kinase activity Inhibits DNA synthesis by competing with POLD3 Play a role in p53 inhibition of cell proliferation in response to DNA damage
Interaction: Nrf2, BCCIP, CIZ1, CUL4A, CCNE1, CDK, DDB1, DTL, GADD45A, GADD45G, HDAC, PCNA, PIM1, TK1, TSG101
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, Cell Proliferation and Viability
Molecular Family: Tumor Suppressors
Antigen References: Sorteberg AL, et al. 2022. Front Oncol. 12:906194. Galanos P, et al. 2016. Cell Cycle. 23:3153-3154. Lai L, et al. 2020. Int J Mol Sci. 21:8504. Xiao BD, et al. 2020. World Stem Cells. 12:481.
Gene ID: 1026
UniProt: View information about p21 on UniProt.org
Clone: W22006E
Regulatory Status: RUO
Other Names: Cyclin Dependent Kinase Inhibitor 1A, CAP20, CIP1, WAF1, SDI1, p21, p21 CIP1, CDKN1, p21 Cip1/Waf1
Isotype: Rat IgG2b, κ